复方枳椇子对四氯化碳致大鼠急性肝损伤的保护作用

目的:研究不同剂量复方枳棋子生粉及水提液对四氯化碳致大鼠急性肝损伤的保护作用.方法:大鼠随机分成6组,空白对照组,复方枳棋子高、中、低剂量生粉组、水提组和模型对照组,每组各10只,雌雄各半.受试组每日按体重ig给予受试样品20 mL·kg-1(水提液剂量560 mg· kg-1；高、中、低剂量生粉组分别为560,420,280 mg· kg -1),空白对照组和模型对照组按20 mL· kg -1体重给予蒸馏水.实验连续30 d,于最后1次ig后,模型组及各受试组按1 mL· kg-1一次皮下注射CCI4原液,观察丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)和肝脏病理变化.结果:模型组与空白对照组比较,ALT,AST及LDH明显升高,差异有显著性意义(P＜0.05).生粉高剂量组与模型组比较,LDH有明显改善,差异有显著性意义(P＜0.05)；生粉中剂量组与模型组比较,ALT,LDH有明显改善,差异有显著性意义(P＜0.05)；水提组与模型组比较,ALT及LDH有明显改善,差异有显著性意义(P＜0.05).肝脏病理切片观察各实验组与模型组比较,各实验组的肝损伤均有减轻的趋势,尤以水提组最明显,差异均有显著性意义(P＜0.05).结论:复方枳棋子对四氯化碳致大鼠急性肝损伤具有一定的保护作用,尤以复方枳棋子水提组最为明显.

The Protection of Compound Recipe Hovenia dulcis on Acute Liver Injury Induced by Carbon Tetrachloride in Rats

Objective: To study the protection of compound recipe Hovenia dulcis powder and water extract groups on acute liver injury induced by carbon tetrachloride (CCl₄) in rats. Method : Sixty male and female rats were devided into six groups randomly, the control group, the high-dose, middle-dose, low-dose of crude powder groups, the aqueous extract of H. dulcis group and model groups. The rats were injected carbon tetrachloride to copy the acute liver injury model, to observe activity of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase(LDH) after oral administrating compound recipe H. dulcis for 30 days. Result : Compared with model group and blank control group, ALT, AST and LDH levels were significantly increased. Compared with model groups in crude powder high-dose group, LDH were significantly improved. In the crude powder middle-dose group, ALT and LDH levels were significantly improved. In the crude powder low-dose group, ALT, AST and LDH levels were significantly improved. In the aqueous extract of H. dulcis group, ALT and LDH levels were significantly improved. The pathological change of liver was observed, compared with model and test groups, liver injury was alleviated by sum analysis of ranks, especially in the aqueous extract of H. dulcis group. Conclusion: Compound recipe H. dulcis shows protective effect against experimental liver injury in rats, especially the the aqueous extract of H. dulcis group has more significant action.