HPLC测定核桃青皮中胡桃醌的含量

目的:建立一种测定核桃青皮中胡桃醌含量的高效液相色谱法,为实现核桃青皮的合理开发利用提供依据.方法:采用高效液相色谱法测定了核桃鲜皮和冷冻青皮中的胡桃醌含量,对此方法进行了方法学评价.结果:胡桃醌对照品在0.01 ～8.00 μg线性相关良好(r=0.9993);检出限为0.195 ng;平均回收率为98.67％ (n =6),RSD＜2.03％；精密度RSD ＜0.58％ (n =7).新鲜核桃青皮中胡桃醌的含量为1.66 mg·g-1,冷冻青皮含量为1.54 mg·g-1.结论:该方法样品前处理简单、分析快速,重复性好,准确度和精密度高,适合胡桃醌类样品的分析测定.

Determination of Juglone in the Husk of Walnut by HPLC

Objective:To establish an HPLC method for determining the content of juglone in the husk of walnut,to provide the basis for the resource exploitation and utilization of juglone.Method:The method was evaluated by HPLC in methodology and the samples which were fresh and stored in freeze temperature were determined.Result: This HPLC methord have a simple preparation for popularization easily.The results indicate that this certain method had good linear relationship under the condition that the content was between 0.01 μg and 8 μg(r=0.999 3) and the detection limit of this method was 0.195 ng.The average recovery was 98.67%(n=6),RSD was below 2.03%;the RSD of the indicated accuracy was below 0.58%(n=7).Juglone in the husk of fresh walnut was 1.66 mg·g-1 and another sample was 1.54 mg·g-1 which was stored in freeze temperature.Conclusion: This certain method is simple,fast and reproducible with a high accuracy and precision,which fit for the analysis of juglone.