毛樱桃总黄酮超声提取工艺优选

目的:优选毛樱桃总黄酮的超声提取工艺。方法:以总黄酮得率为评价指标,紫外分光光度法测定总黄酮含量,采用正交试验法考察提取时间、料液比、乙醇体积分数、超声功率等对毛樱桃总黄酮超声提取工艺的影响,响应曲面法优选提取条件。结果:最佳提取工艺为20倍量95%乙醇于400 W功率下提取45 min。此工艺条件下,总黄酮平均得率1.135%。结论:乙醇超声提取法经济实用、方便快捷,可在中药材提取领域推广使用。

Optimization of Ultrasonic Extraction Technology for Total Flavones from Prunus tomentosa

Objective: To optimize ultrasonic extraction technology of total flavones from Prunus tomentosa.Method: Taking yield of total flavones as evaluation index,the content of total flavones was determined by UV,orthogonal test method was used to optimize ultrasonic extraction technology with extraction time,ratio of solid-liquid,the concentration of ethanol and ultrasonic power as factors,extraction condition of total flavones was optimized by response surface method.Result: The best extraction condition was:the concentration of ethanol 95%,ratio of solid-liquid 1∶20,ultrasonic power 400 W,extractive time 45 min,under this condition,average yield of total flavones could be up to 1.135%.Conclusion: Ethanol ultrasonic extraction was low consumption,common used and convenient,it could be widely used in extraction technology of Chinese herbal medicine.