HPLC-TOF/MS对中药复方扶正平消胶囊化学成分的鉴别

目的 运用高效液相-高分辨飞行时间质谱(HPLC-TOF/MS)技术对中药复方扶正平消胶囊化学成分进行鉴别。方法 色谱分离采用Agilent Eclipse plus C18 (4.6 mm×250 mm,5 μm) 色谱柱；流动相为乙腈和0.1％甲酸水溶液,梯度洗脱:0~90 min,5%A~95%A；柱温25℃；流速1 ml/min,柱后分流比为31。质谱定性采用飞行时间质谱,电喷雾离子源(ESI),正负离子模式共同监测,质量数扫描范围m/z 100~1 100。结果 共鉴别出扶正平消胶囊中247种的化学成分,其中正离子模式下168个、负离子模式下103个、正负离子均有响应24个,并对成分进行了药材归属。结论 建立了一种基于HPLC-TOF/MS技术对中药复方扶正平消胶囊中的化学成分进行鉴别的有效方法,为其质量控制及体内的深入研究奠定了基础。

HPLC-TOF/MS in identification of chemical constituents of Fuzhengpingxiao Capsule

Objective To use high-performance liquid chromatography-time of flight mass spectrometry(HPLC-TOF/MS) for analyzing the chemical constituents of Fuzhengpingxiao Capsule.Methods The separation was performed on a Agilent Eclipse plus C18 reverse phase column(4.6 mm×250 mm,5 μm).The mobile phase consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute.The flow rate was 1 ml/min,the post-column split ratio was 31,and the temperature of column was 25℃.Time-of-flight mass spectrometer(TOF/MS) and electrospray ion source(ESI) was employed for qualitative analysis under both positive and negative ion mode,and mass scan range was m/z 100-1 100.Results A total of 247 major chemical constituents were identified in Fuzhengpingxiao Capsule by HPLC-TOF/MS,including 168 in positive mode,103 in negative mode,and 24 in both.The source herb of the components were identified.Conclusion An efficient HPLC-TOF/MS approach has been established for studying the chemical constituents in Fuzhengpingxiao Capsule,which paves a way for the quality control and further in vivo studies of the preparation.