| 6株抗rhlL－8单克隆抗体识别表位的鉴定及双单克隆抗体夹心法ELISA检测IL－8方法的建立 |
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| 引用本文： | 刘,金伯泉,董邦权,刘雪松,苏丽,周宝宏,马大龙. 6株抗rhlL－8单克隆抗体识别表位的鉴定及双单克隆抗体夹心法ELISA检测IL－8方法的建立[J]. 中国免疫学杂志, 1994, 0(3) |
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| 作者姓名： | 刘 金伯泉 董邦权 刘雪松 苏丽 周宝宏 马大龙 |
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| 作者单位： | 第四军医大学免疫学教研室，北京医科大学免疫学教研室 |
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| 摘 要： | 采用酶标抗体竞争结合试验对６株抗ＩＬ－８单克隆抗体识别的表位进行了鉴定。按识别表位的不同可将６株单克隆抗体分为两组，一组包括２Ｇ２、４Ｃ３和４Ｅ１，识别相同或相近的表位；另一组包括４Ｄ７、５Ｃ９和５Ａ４，识别相同或相近的表位。在表位鉴定的基础上，选择识别不同表位的单克隆抗体用于建立双单克隆抗体夹心法ＥＬＩＳＡ检测ＩＬ－８，其中以４Ｄ７为包被抗体，４Ｃ３为酶标抗体的组合可获得最高的敏感性，对单核细胞源性ｒｈＩＬ－８（ＭＩＬ－８）及内皮细胞源性ｒｈＩＬ－８（ＥＩＬ－８）检测的敏感性可分别达到１５６ｐｇ／ｍｌ及３１２ｐｇ／ｍｌ。
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| 关 键 词： | 白细胞介素－8，酶联免疫吸附试验，单克隆抗体 |
THE CHARACTERIZATION OF EPITOPES RECOGNIZED BY 6MONOCLONAL ANTIBODIES TO IL-8 AND ESTABLISHMENT OFSANDWICH ELISA FOR DETECTION OFIL-8 |
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| Liu Xin,JinBopuan,Dong Bangpuan et al.. THE CHARACTERIZATION OF EPITOPES RECOGNIZED BY 6MONOCLONAL ANTIBODIES TO IL-8 AND ESTABLISHMENT OFSANDWICH ELISA FOR DETECTION OFIL-8[J]. Chinese Journal of Immunology, 1994, 0(3) |
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| Authors: | Liu Xin JinBopuan Dong Bangpuan et al. |
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| Abstract: | pitopes recognized by 6 monoclonal antibodies to IL-8 were characterized by HRP labelledantibody competitive binding assay.It was found that these 6 monoclonal antibodies can be divid-ed into two groups according to the epitopes they recognized.2G2,4C3 and 4E1 belong to a sin-gle group while another group include 4D7,5A4 ang 5C9.On this basis,a sandwich ELISA wasestablished using 4D7 as coating antibody and HRP labelled 4C3 as second antibody with its de-tection limit about l50pg/ml. |
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| Keywords: | BZ-8 ELISA Monoclonal antibody |
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