| 淫羊藿苷对体外培养的成骨细胞骨形态发生蛋白-2表达的影响 |
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| 引用本文： | 柯勇,张功礼,禹志宏,孔霞,郭凌郧,郑飞. 淫羊藿苷对体外培养的成骨细胞骨形态发生蛋白-2表达的影响[J]. 郧阳医学院学报, 2008, 27(3) |
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| 作者姓名： | 柯勇 张功礼 禹志宏 孔霞 郭凌郧 郑飞 |
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| 作者单位： | [1]郧阳医学院附属人民医院创伤骨科,湖北十堰442000 [2]湖北中医学院,湖北武汉430073 [3]郧阳医学院附属人民医院临床医学研究所,湖北十堰442000 |
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| 摘 要： | 目的:检测不同浓度淫羊藿苷(ICA)对体外培养的成骨细胞MC3T3-E1中骨形态发生蛋白-2(BMP-2)表达的影响。方法:分别用10、20、40ng/mL的淫羊藿苷作用MC3T3-E1细胞,观察细胞生长情况;MTT法测绘细胞生长曲线;FCM法测定细胞生长周期;免疫细胞化学方法和Western bloting测定细胞BMP-2蛋白表达情况。结果:淫羊藿苷组可使细胞的生长曲线明显上移,S期细胞百分率升高,G1期细胞百分率减少以及BMP-2蛋白表达量增加,其中20ng/mL组作用更为显著,各组间比较有显著差异(P(0.01)。结论:淫羊藿苷可以促进成骨细胞增殖,增加成骨细胞BMP-2的表达。
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| 关 键 词： | 成骨细胞 骨形态发生蛋白-2 淫羊藿苷 |
Effects of Icariin on the Expression of Bone Morphogenetic Protein-2 in Osteoblasts |
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| KE Yong,,ZHANG Gong-li,YU Zhi-hong,KONG Xia,GUO Ling-yun,ZHENG Fei. Effects of Icariin on the Expression of Bone Morphogenetic Protein-2 in Osteoblasts[J]. Journal of Yunyang Medical College, 2008, 27(3) |
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| Authors: | KE Yong ZHANG Gong-li YU Zhi-hong KONG Xia GUO Ling-yun ZHENG Fei |
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| Affiliation: | KE Yong1,2,ZHANG Gong-li1,YU Zhi-hong1,KONG Xia3,GUO Ling-yun3,ZHENG Fei3 (1Department of Orthopaedics,Renmin Hospital,Yunyang Medical College,2Hubei College of Traditional Chinese Medicine,Wuhan,Hubei 430073,China,3Institute of Clinical Medicine,Shiyan,Hubei 442000,China) |
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| Abstract: | Objective To explore the effect of icariin on the expression of Bone Morphogenetic Protein-2 in MC3T3-E1 cells.Methods MC3T3-E1 cells were incubated with different concentrations of icariin (10 ng/mL,20 ng/mL,and 40 ng/mL,respectively).MTT method was used to observe the cell proliferation.The cell growth cycle was detected by flow cytometry.The expression of BMP-2 in MC3T3-E1 cells was examined by immunohistochemistry staining and western blotting. Results Icariin made the growth curve of MC3T3-E1 cells ele... |
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| Keywords: | Osteoblasts Bone Morphogenetic Protein-2 Icariin |
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