穿膜蛋白1和基质金属蛋白酶9在胆管癌中的表达及意义

目的通过观察胆管癌组织中穿膜蛋白1(DLK1)与基质金属蛋白酶9(MMP-9)的表达,分析两者的临床意义及相关性。方法收集2001-2010年96例胆管癌患者癌组织,20例正常人胆管组织作为对照,采用SP免疫组织化学法检测组织中DLK1与MMP-9的表达。组间比较采用卡方检验,相关性检验采用Spearman相关分析。结果 DLK1与MMP-9在胆管癌中的阳性表达率分别为59.38%和79.17%,2者显著高于在正常胆管组织中的表达(0和15%),差异有统计学意义(χ2=23.35、31.37,P0.05)。胆管癌中DLK1的表达与肿瘤的分化程度相关(χ2=7.46,P0.05);MMP-9的表达与肿瘤的分化程度、淋巴结转移相关(χ2=7.28、9.67,P0.05),与性别、年龄、肿瘤组织大小无关(P0.05)。两者在胆管癌组织中的表达呈正相关(r=0.41,P0.05)。结论 DLK1与MMP-9在胆管癌中高表达,且两者呈正相关,提示可能与胆管癌的发生、发展、侵润、转移有关,其表达的检测为胆管癌的诊断及治疗提供一定的参考依据。

Expression and clinical significance of DLK1 and MMP -9 in cholangiocarcinoma

Objective To investigate the expression of delta drosophila homolog - like 1 （ DLK1 ） and matrix metalloproteinase 9 （ MMP - 9） in eholangiocarcinoma tissue and to analyse their clinical significance and the correlation between DLK1 and MMP- 9. Methods Cholangio carcinoma tissues were collected from 96 patients who were pathologically diagnosed with cholangiocarcinoma from 2001 to 2010, and normal bile duct tissues were collected from 20 normal persons. The expression of DLK1 and MMP - 9 in cholangiocarcinoma tissues or normal bile duct tissues was measured by immunohistochemical S - P method. Chi - square test was performed using SPSS 17.0. Correlation analysis was performed by Speaimang correlation test. Results The positive expression rates of DLK1 and MMP - 9 in cholangiocarcinoma tissues were 59. 38% and 79.17%, respectively, significantly higher than those in normal bile duct tissues （0 and 15% ） （X^2 = 23.35, P 〈 0. 05; X^2 = 31.37, P 〈 0.05 ）. The expression of DLK1 was correlated with the differentiation degree in cholangiocarcinoma （X^2 = 7. 46, P 〈 0. 05 ） ; the expression of MMP -9 was correlated with the differentiation degree and lymph node metastasis in cholangiocarcinoma （X^2 = 7. 28, P 〈 0. 05; ）（2 =9. 67, P 〈 0.05 ）, but was not correlated with sex, age, and tumor size （P 〉 0.05 ）. There was a positive correlation between the expres sion of DLK1 and MMP - 9 （ r = 0. 41, P 〈 0.05 ）. Conclusion DLK1 and MMP - 9 are highly expressed in cholangiocarcinoma, and they arc positively correlated, suggesting that they arc associated with the development, progression, invasion, and metastasis of cholangiocarcinoma. The detection of the two indicators provides a reference for the diaguosis and treatment of eholangiocarcinmna.