旋磁场对大鼠肾、脑组织自由基代谢的影响

目的:研究旋磁场对大鼠肾组织、脑组织中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和一氧化氮(NO)含量的影响.方法:用邻苯三酚自氧化法测定SOD活力;TBA显色法测定MDA含量;改良的Griess法测定NO含量.结果:在30mT磁场中曝磁30min,大鼠肾组织和脑组织中SOD活力高于对照组;大鼠肾组织MDA含量显著低于对照组,脑组织MDA含量虽低于对照组,但差异无统计学意义;大鼠肾组织NO含量显著高于对照组,脑组织NO含量无明显变化.结论:旋磁场可影响大鼠肾、脑组织的自由基代谢.

The Effect of Rotating Magnetic Field on the Metabolism of Free Radicals in the Kidney and Brain Tissue of Rat

Objective:To study the effect of rotating magnetic field on the activity of superoxide dismutase (SOD) , and the contents malon-dialdehyde (MDA) and nitric oxide (NO) in the kidney and brain tissues of rat. Methods: SOD activity is determined with Trihy droxy benzene; MDA ,with TBA (Thih bar bituric acid);and NO content,with improved Griess method.Results:After 30-minute exposure to a 30mT magnetic field,the activity of SOD in the kidney and brain tissues of rats in the experimental group is higher than that in the control group .The content of MDA in the kidney tissues of rats is significantly lower than that in the control group.Although the content of MDA in the brain tissues is lower than than in the matched group,there is no statistical significance. The content of NO in the kidney tissues of rat is notably higher than that in the matched group .The contents of NO in the brain tissues in the two groups have no remarkable difference. Conclusion: The rotating magnetic field could affect the metabolism of free radicals.