可诱导共刺激分子在系统性红斑狼疮患者外周血T淋巴细胞的表达

目的 探讨可诱导共刺激分子(ICOS)在系统性红斑狼疮(SLE)患者外周血T淋巴细胞的表达。方法 应用双色荧光抗体染色技术经流式细胞仪检测了33例SLE患者和16例正常人对照者外周血中T淋巴细胞表面ICOS的自然表达水平,同时收集SLE患者的实验室检查指标,并用SLE疾病活动指数(SLEDAI)来判定SLE患者疾病活动程度,比较分析不同组别SLE患者ICOS表达水平与SLEDAI的相关性。结果 活动期SLE组T淋巴细胞ICOS表达水平明显高于正常人对照组(P<0.01)和非活动期SLE组(P<0.01),非活动期SLE组与正常人对照组T淋巴细胞ICOS表达水平则差异无统计学意义(P>0.05)。活动期组与非活动期组SLE患者T淋巴细胞ICOS表达水平均与SLEDAI呈显著正相关(r=0.71,P=0.001、r=0.56,P=0.03)。结论 活动期SLE患者T淋巴细胞ICOS表达增高,ICOS可能与SLE的发病机制有关。

Expression of Inducible Co-Stimulator on T lymphocytes in Peripheral Blood from the Patients with Systemic Lupus Erythematosus

Objective To investigate the expression of inducible co-stimulator (ICOS) on T lymphocytes in peripheral blood from the patients with systemic lupus erythematosus (SLE). Methods Two-color immunofluorescent staining and flow cytometric assay were used to analyze the level of ICOS expression on T lymphocytes in peripheral blood from 33 SLE patients and 16 healthy volunteers. The data of systemic lupus erythematosus diseases activity index (SLEDAI) in SLE patients were also collected. Results The level of ICOS expression on T cells from active SLE group was markedly higher than that in the control group and the inactive SLE group (P<0.01). But there was no significant difference between the inactive SLE group and the control group (P>0.05). However, a positive correlation between the expression level of ICOS and the SLEDAI score was found in both active SLE group and inactive SLE group(r=0.711,P=0.001、r=0.561,P=0.03). Conclusions The expression of ICOS on T cell in peripheral blood from active SLE is increased, which might play a role in the pathogenesis of SLE.