| 利用基因芯片研究无精子症相关基因及RAP1 A基因初步探讨 |
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| 引用本文： | 杨波,高晓康,王禾,刘贺亮,陈宝琦,袁建林,邵国兴,邵晨. 利用基因芯片研究无精子症相关基因及RAP1 A基因初步探讨[J]. 中华男科学杂志, 2003, 9(9): 654-657 |
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| 作者姓名： | 杨波 高晓康 王禾 刘贺亮 陈宝琦 袁建林 邵国兴 邵晨 |
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| 作者单位： | 第四军医大学西京医院泌尿外科,陕西,西安,710032 |
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| 基金项目： | 陕西省人口与计划生育专项课题(200102) |
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| 摘 要： | 目的 :应用基因芯片技术获取正常生育人睾丸组织与无精子症病人睾丸组织中差异表达的基因 ,并对结果进行初步分析研究。 方法 :抽提正常生育人睾丸组织与无精子症病人睾丸组织中的mRNA来制备探针 ,经过点样、杂交及洗涤后 ,通过计算机观察两者表达谱的差异情况 ,随后通过文献检索系统对其中明显上调的基因之一RAP1A进行了初步分析。 结果 :通过基因芯片筛选 ,获得 6 2 3条差异表达的基因 ,其中明显上调的基因RAP1A与人类精子调控关系密切。 结论 :基因芯片筛选正常生育人睾丸组织与无精子症病人睾丸组织差异表达的基因具有样品用量少、速度快的特性 ,可用于高通量筛选无精子症基因以及进一步的研究。
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| 关 键 词： | 无精子症 基因芯片 RAP1 A |
| 文章编号： | 1009-3591(2003)09-0654-04 |
| 修稿时间： | 2003-02-10 |
A Study of Aspermia-related Genes by Genchips and Analysis of the RAP1A Gene |
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| Bo YANG,Xiao Kang GAO,He WANG,He Liang LIU,Bao Qi CHEN,Jian Lin YUAN,Guo Xing SHAO,Chen SHAO. A Study of Aspermia-related Genes by Genchips and Analysis of the RAP1A Gene[J]. National journal of andrology, 2003, 9(9): 654-657 |
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| Authors: | Bo YANG Xiao Kang GAO He WANG He Liang LIU Bao Qi CHEN Jian Lin YUAN Guo Xing SHAO Chen SHAO |
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| Affiliation: | Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. |
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| Abstract: | Objective: To study the differential gene expression profiles between the normal and aspermia human testes by genechips.Methods: Probes were prepared from mRNA extracted from both normal and aspermia testes and employed on Biostar H 40s genechips to detect the differential gene expression profiles. A distinctly up regulated gene RAP1A was analyzed by bibliographic retrieval. Results: Six hundred and twenty three differential expressed genes were found, among which the distinctly up regulated gene RAP1A was closely related to human sperm regulation. Conclusions: Screening the differential gene expression profiles between the normal and aspermia human testes by genechips can be used in the study of aspermia related genes. |
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| Keywords: | Aspermia Genechips RAP1 A |
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