丹参素冰片酯乳剂中丹参素冰片酯和冰片的含量测定

目的 建立可行的丹参素冰片酯(DBZ)乳剂中DBZ和冰片的含量测定方法.方法 采用高效液相色谱法测定丹参素冰片酯,色谱柱采用Agilent TC-C18柱(250 mm×4.6 mm,5 μm),以甲醇-0.2%甲酸水(65:35)为流动相,检测波长为280 nm.采用气相色谱法测定冰片.HP-1毛细管柱(30.0 m×0.32 mm×0.25μm).进样口温度为260℃,检测器温度为260℃.结果 丹参素冰片酯与杂质峰分离良好,线性范围为0.01-1.00 g/L(R2=0.999);乳剂样品中丹参素冰片酯的含量为4.75 g/L,冰片与杂质峰分离良好,质量浓度线性范围为0.001-0.050 g/L(R2=0.999),乳剂样品中冰片未检出.结论 所采用方法准确、灵敏、专属性好,可用于丹参素冰片酯乳剂中丹参素冰片酯和冰片含量的测定.

Determinatation of Tanshinol Borneol Ester and D-Borneol in the Emulsion of Tanshinol Borneol Ester

Objective To determine tanshinol borneo1 ester and D- borneol in the Tanshinol Borneol Ester Emulsion. Methods DBZ is determinated by HPLC method. The column is Agilent TC-C18（250 mm×4.6 mm,5μm） and the mobile phase is the mixed solution of methanol and 0.2% formic acid water （65：35）. The detection wavelength was 280 nm. Borneol was determined by GC. The column is HP - 1 capillary column （30. 0 m × 0. 32 mm × 0. 25 μm） and both of injection and detection temperature is 260 ℃. Results The main peak of DBZ is divided well with other impurity and there is a good linear range between 0. 01- 1.00g/L（R2 =0. 999）. The content of DBZ in the sample of DBZ emulsion is 4. 75 g/L. The main peak of D- borneol is divided well with other impurity and there is a good linear range between 0.001-0.050 g/L（R2=0.999）.The content of D-borneol is not arrayed in the sample of DBZ emulsion. Conclusion The method was accurate, sensitive, specific, and can be used for determination of DBZ and D- borneol in the DBZ emulsion.