高效液相色谱法测定大鼠血清中盐酸柔红霉素浓度

目的 建立测定大鼠血清中柔红霉素血药浓度的高效液相色谱法.方法 色谱柱为Phenomenex C18柱(250mm×4.6mm,5 μm),流动相为甲醇-0.01 ml/L磷酸二氢铵溶液(pH=4.65)-冰醋酸(30:20:0.1),流速1.0 mL/min,检测波长233 nm,柱温35 ℃,内标为盐酸多柔比星,进样量20 μL.结果 盐酸柔红霉素质量浓度在0.147 8～14.77 8 mg/L(r=0.998 4)范围内与待测物和内标峰高比值线性关系良好,低、中、高3种质量浓度(0.738 9,3.694 5,14.778 0 mg/L)的平均相对回收率分剐为102.5%,98.7%,99.3%,日内、日间RSD均小于10%.结论 该方法样品处理简便,可用于血清中盐酸柔红霉素的血药浓度测定和药代动力学研究.

Determination of Concentration of Daunorubicin Hydrochloride in Rat Serum by HPLC

Objective To establish the determination method of serum concentration of daunorubicin hydrochloride by HPLC.Methods The samples were separated on the Phenomenex C18 column（250 mm×4.6 mm,5 μm） with the mobile phase consisted of methanol-0.01 mol/L ammonium dihydrogen phosphate solution（pH=4.65）-glacial acetic acid（30 ∶20 ∶0.1） at the flow rate of 1.0 mL/min.The UV detection wavelength was 233 nm.The column temperature was 35℃.The internal standard was doxorubicin hydrochloride.The injection volume was 20 μL.Results The linear range for daunorubicin hydrochloride was 0.147 8-14.77 8 mg/L（r=0.998 4）.The mean relative recovery rates of daunorubicin hydrochloride at low,middle and high concentrations（0.738 9,3.694 5,14.778 0 mg/L）were 102.5%,98.7%and 99.3%,respectively.Both intra-day and inter-day RSD were below 10%.Conclusion This method is simple and suitable for blood concentration monitoring and pharmacokinetic study of daunorubicin hydrochloride.