三肽化合物酪丝亮肽(YSL)对腹水型肝癌H_(22)小鼠免疫调节作用实验研究

目的观察三肽化合物酪丝亮肽(tyroserleutide,YSL)对腹水型肝癌H22小鼠的抗肿瘤作用,观察YSL对荷瘤小鼠T淋巴细胞转化及NK细胞杀伤活性的影响。方法建立腹水型肝癌H22小鼠动物模型,观察YSL对腹水型肝癌H22小鼠生存时间的影响;以MTT法检测YSL对荷瘤小鼠T淋巴细胞转化及NK细胞杀伤活性的影响。结果YSL能够明显延长腹水型肝癌H22小鼠生存时间;YSL在5μg.kg-1和50μg.kg-1时可以促进腹水型肝癌H22小鼠T淋巴细胞的转化;YSL在0.5、5和50μg.kg-1时增强荷瘤小鼠NK细胞杀伤活性。结论YSL可以延长腹水型肝癌H22小鼠的生存时间;促进荷瘤鼠的T淋巴细胞转化及NK细胞杀伤活性。

Immunological regulation of tripeptide tyroserleutide (YSL) on mice bearing ascitic fluid-type hepatocarcinoma H22

Aim This study aimed to observe effects of tyroserleutide(tyrosyl-seryl-leucine,YSL) on the survival time of mice transplanted with the ascitic fluid-type hepatocarcinoma H_(22),as well as the T lymphocyte transformation and killing activity of NK cell impacted by YSL on mice bearing H_(22) tumor.Methods The model of ascetic fluid-type hepatocarcinoma H_(22) was established and the survival time of mice bearing H_(22) tumor treated by YSL was observed.MTT was used to observe the effect of lymphocyte transformation and killing activity of NK cells activated by YSL in vitro.Results YSL could significantly prolong the survival time of mice bearing ascetic fluid-type hepatocarcinoma H_(22).At doses of 5 and 50 μg·kg~(1),YSL could advance the T lymphocyte transformation.At doses of 0.5,5 and 50 μg·kg~(-1),YSL could enhance the killing activity of NK cells on mice bearing H_(22) tumor.Conclusion YSL can significantly prolong the survival time of mice bearing fluid-type hepatocarcinoma H_(22) and promote the effect of T lymphocyte transformation and NK cell killing activity.