HPLC法测定中成药中9种化学降糖药的研究

目的：建立测定添加在降糖中成药中的格列吡嗪、格列本脲、格列齐特、格列喹酮、格列美脲、盐酸吡格列酮、盐酸罗格列酮、那格列奈、瑞格列奈共9种化学降糖药的高效液相分析方法。方法：色谱柱：ThermoC18（150mm×4．6mm，5μm），流动相：醋酸盐缓冲溶液（10mmol·L-1醋酸铵和20mmol·L-1三乙胺，醋酸调pH至5．5）-乙腈（61：39），流速：1ml·min-1，检测波长：210nm，柱温：30℃，进样20山。结果：9种化学降糖药完全分离，检出限为4～18ng；9种药物具有宽的线性范围和良好的线性关系（r≥0．9994）；日内、日问RSD分别为O．09％～1．42％和O．30％～1．88％；在中药中的加样回收率除个别药物低浓度外均大于80％，RSD为0．26％-5．91％。结论：该方法用于检测中成药中的化学降糖药快速、简便、高效。

Simultaneous Determination of Nine Anti-diabetic Drugs in Chinese Traditional Patent Medidnes by HPLC

Objective： To establish the HPLC method for the simultaneous determination of nine anti-diabetic agents in chinese traditional patent medicines. The chemical anti-diabetic agents were glipizide, glibenclamide, gliclazide, gliquizone, glimepiride, piolita- zone hydrochloride, rosiglitazone hydrochloride, nateglinide, repaglinide. Method： The Thermo Cls analytical column （ 150 mm x 4.6 mm,5txm） was used,the mobile phase was acetonitrile-acetate buffer（ 10 mmol-L-1 ammonium acetate and 20 mmol. L-ltriethyl- amine, the pH of solution was adjusted to 5.5 using acetic acid） （61： 39）, the temperature was 30 ~C. Result： The lowest detectable limits were 4-18 ng, the linear relation and range of each component was obtained, and correlation coetticients were better within their ranges （r~〉0. 999 4）. Intra-day and inter-day relative standard deviations（RDSs） were 0. 09%-1.42% and 0. 30%-1.88% respec- tively. The average recoveries in different kinds of chinese traditional patent medicine were more than 80% except for the low concentra- tion of glimepiride was 46.5% ,RSDs of recoveries were 0. 26% -5.91%. Conclusion： The study reveals the method can be used in analysis of anti-diabetic drugs in chinese traditional patent medicine, and it is rapid, convenient, economic specific and accurate.