E26转录因子与基质金属蛋白酶9在脑膜瘤中的表达及相关性研究

目的研究E26转录因子(Ets-1)和基质金属蛋白酶9(MMP-9)在脑膜瘤中的表达及相关性。方法采用免疫组织化学法检测56例脑膜瘤石膜标本(良性组39例、恶性倾向组17例)中Ets-1和MMP-9的表达,采用逆转录聚合酶链反应(RT-PCR)方法检测30例新鲜脑膜瘤(良性组22例,恶性倾向组8例)中Ets-1和MMP-9的mRNA水平。另取9例行脑外伤减压术的正常脑组织作为对照组结果正常脑组织Ets-1和MMP-9不表达或低表达,脑膜瘤中Ets-1阳性表达率48.2%(27/56),MMP-9阳性表达率62.5%(35/56);脑膜瘤恶性倾向组Ets-1和MMP-9的阳性表达率高于脑膜瘤良性组(P<0.05);Ets-1和MMP-9在脑膜瘤中的表达呈正相关(rs=0.284,P<0.05)。脑膜瘤中Ets-1和MMP-9的mRNA含量高于正常脑组织(P<0.05),脑膜瘤恶性倾向组Ets-1和MMP-9的mRNA含量高于脑膜瘤良性组(P<0.05),Ets-1和MMP-9在脑膜瘤的mRNA水平呈正相关(r=0.479、P<0.02)。结论Ets-1和MMP-9的表达与脑膜瘤恶性度有关,两者在脑膜瘤发生、发展过程中有协同作用,可作为脑膜瘤预后的潜在指标。

Expression of and correlation between E26 transformation-specific 1 and matrix metalloproteinases 9 in meningioma

Objective To study the expression of E26 transformation-specific 1(Ets-1) and matrix metalloproteinase 9(MMP-9) in meningioma and the correlation between Ets-1 and MMP-9.Methods The expressions of Ets-1 and MMP-9 were detected by immunohistochemistry in 56 paraffinaceous specimen with meningioma,including 39 with benign tumor and 17 with malignant tendency.RT-polymerase chain reaction(RT-PCR) was used in 30 cases of fresh meningioma(22 benign and 8 with malignant tendency).The 9 normal brain tissues subjected to cerebral trauma were control group.Results The normal brain tissue was negative or only had a low expression for Ets-1 and MMP-9.48.2%(27/56) and 62.5%(35/56) of meningiomas were positive for Ets-1 and MMP-9 respectively.The positivity rates of Ets-1 and MMP-9 were significantly higher in the malignant tendency group than in the benign group(P <0.05).The Ets-1 positive reaction was correlated with MMP-9(rs=0.284,P <0.05).The contents of Ets-1 and MMP-9 mRNA were significantly higher in meningioma tissues than in the normal brain tissue(P <0.05) and also higher in the malignant tendency group than in the benign group(P <0.05).The Ets-1 mRNA level correlated with MMP-9 mRNA level(r = 0.479,P <0.02).Conclusion The expressions of Ets-1 and MMP-9 are related to the malignancy of meningioma,and they may coordinate with each other in the development and progression of meningioma,and thus could be used as potential marker for the prognosis of meningioma.