研究药物对肿瘤细胞代谢的新方法──灌注细胞31P核磁共振谱

为连续观察药物对细胞代谢的影响,用含紫杉醇的灌注液对人口腔上皮癌KB细胞和人结肠癌HCT-8细胞在灌注条件下进行了细胞³¹P谱测定,从两种细胞在未给药、给药以及停药后的灌注条件下获得的代谢图谱中发现,紫杉醇使KB细胞和HCT-8细胞的ATP代谢水平均有升高,但KB细胞的ATP升高更为明显;同样剂量的长春新碱使KB细胞代谢中的ATP峰的改变不明显,推测可能与它们的作用机制不同有关。研究结果表明,灌注细胞³¹P谱测试法对观察药物对细胞代谢的作用是独特的。

A new method for study of the effect of drugs on cancer cells--31PNMR with perfused cell system]

31P NMR was performed for the studies on the metabolic changes of endogenous phosphorus-containing molecules in KB and HCT-8 cells exposed to taxol at a concentration of 10(-6) mol.L-1. Using the perfusion method, the cells can be detected for a longer time by NMR, so as to give continuous spectra of the two cell-lines during the perfusion with and without the drug. The spectra showed that the levels of ATP peaks for both cells enhanced during the perfusion with the drug, but the change of the level is more prominent in KB cells than in HCT-8 cells. This shows that KB cells are more sensitive to taxol than HCT-8 cells. This is in coincident with the result in the cytotoxicity studies. However, the experiment using vincristine at the same concentrations demonstrated that the level of the ATP peak was not changed significantly.