| 白三烯类化合物(Leukotrienes)对小鼠腹腔巨噬细胞生成白细胞介素6的影响 |
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| 引用本文： | 钟淼,程桂芳,王文杰,周龙恩,朱秀媛,张均田.白三烯类化合物(Leukotrienes)对小鼠腹腔巨噬细胞生成白细胞介素6的影响[J].药学学报,1998,33(5):326-329. |
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| 作者姓名： | 钟淼 程桂芳 王文杰 周龙恩 朱秀媛 张均田 |
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| 作者单位： | 中国医学科学院 |
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| 摘 要： | 探讨了白三烯B4(LTB4)、白三烯C4(LTC4)及白三烯D4(LTD4)对小鼠腹腔巨噬细胞分泌白细胞介素6(interleukin6,IL-6)的影响。用IL-6依赖细胞株B9的MTT方法测定样品中IL-6的含量。结果显示,LTB4能剂量依赖性地增加巨噬细胞培养上清液中IL-6的含量。LTC4及LTD4促进巨噬细胞培养上清液中IL-6含量的最适浓度分别为:6.9×10-8和8.05×10-8mol·L-1。结果提示:LTB4与LTC4及LTD4在某些生物学功能方面有一致性。与LTC4及LTD4相比,LTB4促进巨噬细胞培养上清液中IL-6的含量的最适浓度则要大1～2个数量级,提示在炎症反应中,肽白三烯与IL-6的相关性较强。
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| 关 键 词： | 白三烯 白细胞介素6 B9细胞株 |
| 收稿时间： | 1997-07-21 |
EFFECTS OF LEUKOTRIENES ON PRODUCTION OF INTERLEUKIN 6 FROM MOUSE PERITONEAL MACROPHAGES |
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| M Zhong,G Cheng,W Wang,L Zhou,X Zhu,J Zhang.EFFECTS OF LEUKOTRIENES ON PRODUCTION OF INTERLEUKIN 6 FROM MOUSE PERITONEAL MACROPHAGES[J].Acta Pharmaceutica Sinica,1998,33(5):326-329. |
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| Authors: | M Zhong G Cheng W Wang L Zhou X Zhu J Zhang |
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| Institution: | Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050. |
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| Abstract: | In the present study, effects of leukotrienes on IL-6 production by mouse resident peritoneal macrophages were explored with a bioassay method involving IL-6 dependent murine hybridoma B9 cell line. The results showed that LTB4, LTC4 and LTD4 can enhance the production of IL-6 from cultured peritoneal macrophages of mice. The optimal concentractions for LTB4, LTC4 and LTD4 were found to be 1.45 x 10(-6), 6.9 x 10(-8) and 8.05 x 10(-8) mol.L-1, respectively. The results suggest that peptide leukotrienes might play important role on IL-6 production in the local milieu of inflammation. |
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| Keywords: | Interleukin 6 B9 cell line Leukotrienes |
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