大黄素对豚鼠结肠带平滑肌细胞钾通道活性的影响

用检测平滑肌细胞电活动和张力技术,研究了大黄素对豚鼠结肠带平滑肌细胞电和收缩活动的影响并与 cromakalim, glybenclamide,四乙胺及BaCl₂的作用进行比较。结果表明,大黄素加强平滑肌细胞电和收缩活动,作用效果与剂量有关;大黄素与cromakalim的作用相互抑制。其促进平滑肌细胞电和收缩活动的作用与glybenclamide相似,而与四乙胺和BaCl₂有明显区别。提示大黄素的作用机制与抑制细胞膜K_ATP等钾通道的活性相关。

EFFECTS OF EMODIN ON THE ACTIVITY OF K CHANNEL IN GUINEA PIG TAENIA COLI SMOOTH MUSCLE CELLS

The effects of emodin, cromakalim (KATP channel opener) glybenclamide (KATP channel inhibitor) Ba2+ and TEA on the electrical and contractive activities of the smooth muscle cells in guinea pig taenia coli and the relationship between emodin and four other drugs were studied by using intracellular microelectrode technique and the tension measuring technique. The results are as follows: (1) Emodin enhances the electrical and contractive activities of the smooth muscle cells in guinea pig taenia coli. The effects of emodin depend on its concentration. (2) Emodin can resist the inhibition of cromakalim on the electrical and contractive activities of smooth muscle cells. On the other hand, cromakalim was shown to suppress the effects of emodin. (3) Ba2+, TEA and glybenclamide all can improve the electrical and contractive activities of smooth muscle cells and resist the inhibition of cromakalim. The effects of emodin were found to be similar to that of glybenclamide but different from that of Ba2+ and TEA. The results suggest that the mechanism of action of emodin is to inhibit the activity of KATP channel in the guinea pig taenia coli smooth muscle cells.