从海南哥纳香中分离的一种新化合物──海南哥纳香醇甲的抗肿瘤作用

体外用细胞生长曲线测定法、MTT试验、软琼脂集落形成分析法,及体内对移植性肿瘤实验,研究了海南哥纳香醇甲(GHM-10)的抗肿瘤作用,结果表明:GHM-10对肿瘤细胞有较强的抑制作用,IC₅₀在2μg·ml^-1左右;对正常细胞影响较小,骨髓祖细胞的敏感性则更低;耐药的KB/VCR200细胞及其亲本KB细胞具有相似的敏感性。GHM-10对HL-60细胞无分化诱导作用。GHM-10对实体型肝癌H22小鼠、Lewis肺癌小鼠及腹水型S180小鼠均有明显的治疗作用。

STUDIES ON THE ANTICANCER EFFECT OF HOWIINOL A,A NEW COMPOUND ISOLATED FROM GONIOTHALAMUS HOWII

The effect of howiinol A (GHM-10) on the growth of several cancer and normal cells were studied, using the methods of cell growth curve determination, MTT test and soft-agar colony formation assay. The results showed that GHM-10 exhibited potent inhibitory effect on cancer cells with an IC50 of 2 micrograms.ml-1 approximately. Normal cells, especially bone marrow progenitor cells, were shown to be less sensitive to GHM-10. A drug-resistant cell line, KB/VCR200, was found to be as sensitive as its parental KB cells. GHM-10 did not induce HL-60 cell differentiation. In murine transplantable tumors, GHM-10 was found to have significant therapeutic effect on mice bearing solid tumor hepatoma H22, Lewis lung carcinoma and S180 ascites.