| 掌叶大黄毛状根培养及培养物中蒽醌类成分分析 |
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| 引用本文： | 常振战,果德安,沈昕,王沙生,郑俊华.掌叶大黄毛状根培养及培养物中蒽醌类成分分析[J].药学学报,1998,33(11):869-872. |
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| 作者姓名： | 常振战 果德安 沈昕 王沙生 郑俊华 |
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| 作者单位： | 北京医科大学生化及分子生物学系，北京林业大学生物学院 |
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| 摘 要： | 大黄是我国特产的世界著名的传统中药，历代本草均有记载，始载于《神农本草经》，“味苦寒，有毒。主下瘀血、血闭、寒热，破征瘕积聚、留饮宿食，荡涤肠胃，推陈致新，通利水谷，调中化食，安和五脏”。作者已于近期报道了天山大黄发根的诱导方法［１］掌叶大黄Ｒｈｅｕ...
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| 关 键 词： | 掌叶大黄 毛状根 蒽醌类化合物 |
| 收稿时间： | 1997-11-26 |
ANTHRAQUINONE PRODUCTION AND ANALYSIS IN THE HAIRY ROOT CULTURES OF RHEUM PALMATUM L. |
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| Chang Zhenzhan.ANTHRAQUINONE PRODUCTION AND ANALYSIS IN THE HAIRY ROOT CULTURES OF RHEUM PALMATUM L.[J].Acta Pharmaceutica Sinica,1998,33(11):869-872. |
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| Authors: | Chang Zhenzhan |
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| Institution: | Department of Biochemistry and Molecular Biology, Beijing Medical University, Beijing 100083. |
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| Abstract: | Hairy root culture of the medicinal plant Rheum palmatum L. was established by genetic transformation with Agrobacterium rhizogenes. The effects of various media with different pH on growth of the hairy roots and biosynthesis of free anthraquinones were investigated. The experimental results showed that MS agar medium with pH 5.5-5.8 is suitable for growth of the hairy roots. Dark condition is favourable and 62.5-fold increase in fresh weight was reached within a culture period of 25 days. Auxin (0.1 mg.L-1 IAA) activated the hairy root growth but inhibited the biosynthesis of free anthraquinones. About 28% of the total free anthraquinones was released into the liquid medium from the rhubarb hairy roots. |
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| Keywords: | Rheum palmatum L Hairy roots Anthraquinones |
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