人参皂甙Rb1对应激性性行为缺损的保护作用及机制

用悬吊应激模型,小鼠连续应激小鼠10d,每天应激一次,并循序增加应激强度,每次应激前30min,ip人参皂甙Rb₁,观察人参皂甙Rb₁对应激性性行为低下的保护作用。结果表明,应激模型组小鼠性行为明显减少,甚至达到缺损的程度,同时血浆睾酮水平明显降低,而Rb₁各剂量组(2.5,5,10mg·kg^-1)对应激引起的性行为低下及血浆睾酮水平下降均有明显的对抗作用。提示人参皂甙Rb1对应激性性行为低下有保护作用,其机制可能与Rb₁抑制应激动物血浆皮质酮升高和提高睾酮水平有关。

Effect of ginsenoside Rb1 on repeated stress-induced sexual deficiencies in male mice]

The effect of ginsenoside Rb1 has been studied on sexual deficiencies induced by repeated hanging stress. Male mice were stressed by hanging once daily(9:00 am-2:00 pm) for 10 days(1-3 day hung for 2 h, 4-6 day hung for 3 h, 7-9 day hung for 4 h, 10-11 day hung for 5 h). On day 10, they were exposed to female mice treated with estradiol and progesterone and their sexual behaviors (licking, mounting, mating) were assessed at 7:00-9:00 pm. The repeated hanging stress was found to reduce sexual behaviors and decrease plasma testosterone level in mice. Treatments with ginsenoside Rb1(2.5, 5, 10 mg.kg-1, i.p.) 30 min before each stress prevented the repeated stress-induced sexual deficiencies and raised plasma testosterone level. The mechanism of the protective action of ginsenoside Rb1 may be attributed to its action in maintaining normal plasma testosterone level.