2006年四川省部分地区霍乱弧菌毒力基因检测和脉冲场凝胶电泳分型分析

目的分析四川省2006年霍乱弧菌分子特征,分析疫情分离的菌株与监测分离的霍乱弧菌之间的遗传相关性,查找霍乱传染来源,为进一步研究本菌的流行规律和制定防治措施提供依据。方法PCR检测霍乱弧菌毒力基因(ctxAB);脉冲场凝胶电泳(PFGE)对菌株进行分子分型,以BioNumericsV4.0软件UPGMA方法进行聚类分析。结果所试18株霍乱弧菌均具有ctxAB,为产毒株。对18株菌以NotⅠ酶切后PFGE可分为4个型别。结论首次从牛蛙中分离到产毒株。甲鱼中分离的霍乱弧菌与霍乱疫情分离菌株之间同源,被污染的甲鱼可能是本年度食源性霍乱暴发的传染来源之一,应进一步加强对各类海、水产品的监测。

Analysis on Toxin Gene and Pulsed-Field Gel Electrophoresis of Cholera of Sichuan Province in 2006

Objective To study the molecular characteristics of cholera strains collected in Sichuan Province in 2006, to analyze the phylogenic relationship between the strains isolated from sea food samples and strains from outbreaks, to trace the source of cholera infection and so as to study the epidemic trend and support the policy-making on cholera control.Methods Cholera toxin gene was detected by PCR. Pulsed-Field Gel Electrophoresis (PFGE) was used for subtyping of isolates. Clustering of patterns was analyzed with software BioNumerics V4.0. Results In all the analyzed 18 strains, all were toxigenic. All 18 strains were clustered into 4 PFGE patterns when digested with Not I. The patterns of toxigenic strains isolated from turtles in the markets were identical with the patterns of epidemic strains in the same period. Conclusion Strains from turtle are homology with epidemic strains, which points out turtle might be one of the sources of outbreaks. We should strengthen surveillance for sea food and aquatic food.