纤维蛋白原测定系统的探讨

目的　探讨纤维蛋白原 (FIB)冯·克劳斯法 (vonClauss)测定仪器、试剂、标准曲线的配套问题 ,为上海市地区性血液凝固质量管理工作提供依据。方法　使用仪器 试剂 标准曲线配套和非配套的方法对新鲜血浆和不同品牌质控血浆的FIB含量进行测定。结果　使用仪器 试剂 标准曲线配套组合测定新鲜血浆和配套质控血浆的FIB含量结果良好。在仪器 试剂 标准曲线非配套组合时 ,测定新鲜血浆和质控血浆的FIB含量结果有差异。结论　仪器间的FIB标准曲线不能混用 ,仪器 试剂应配套使用。应重视基质效应问题 ,FIB应根据仪器、试剂、方法进行分组统计

Study on the determination system of fibrinogen

Objective To investigate the effect on the fibrinogen(FIB) determination by using different instruments, reagents and standard curve. Methods Use the matched or non matched method with instrument reagent standard curve to determine FIB contents of fresh plasma and quality control(QC) plasma. Results No significant difference of FIB contents in fresh plasma and QC plasma when matched instrument reagent standard curve were used. There is significant difference in the case of using on non matched instrument reagent standard curve. Conclusions Different instruments can not use the same standard curve. Same brand instrument should use matched reagent. Different quality control material had different matrix effect.