| 正交设计优选菟丝子中总黄酮的提取工艺 |
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| 引用本文： | 陈丽,刘密,南希,张燕萍,罗秋爽,李乐.正交设计优选菟丝子中总黄酮的提取工艺[J].数理医药学杂志,2011,24(4):452-453. |
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| 作者姓名： | 陈丽 刘密 南希 张燕萍 罗秋爽 李乐 |
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| 作者单位： | 石河子大学药学院,石河子,832000 |
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| 基金项目： | 石河子大学第九期大学生研究训练计划;项目编号SRP2011228 |
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| 摘 要： | 目的:确定菟丝子总黄酮的最佳回流提取工艺。方法:采取正交优化实验,以芦丁为对照品,采用紫外分光光度法对提取物中的总黄酮含量进行测定。结果:检测波长为510nm,芦丁对照品在0.095~0.475mg/mL内线性良好(r=0.9992),最佳提取工艺条件为提取时间150分钟,提取次数3次,醇浓度为50%,料液比为1:8。结论:该方法稳定,经济,工艺合理。
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| 关 键 词： | 菟丝子 总黄酮 回流提取 正交试验 |
Orthogonal Design for Optimization of Extracting Procedure for Total Flavonoids from Semen Cuscutae |
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| Chen Li,et al.Orthogonal Design for Optimization of Extracting Procedure for Total Flavonoids from Semen Cuscutae[J].Journal of Mathematical Medicine,2011,24(4):452-453. |
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| Authors: | Chen Li |
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| Institution: | Chen Li,et al(Pharmacy College of Shihezi University,Shihezi 832000) |
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| Abstract: | Objective: To study the extraction of flavonoids with the technology of refluxing in Semen Cuscutae.Methods: Rutin as the standard and using ultraviolet spectrophotometry to determine the content of total flavones in semen cuscutae.Results: The linear relateionship of rutin was good from 0.095 to 0.475mg/ml(r=0.9992),The best extraction condition was extraction time 150 minutes,extraction frequency 3 times,alcohol concentration 50%,material-liquid 1:8.Conclusion: The method is accurate,reliable and stable. |
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| Keywords: | Semen cuscutae total flavonoids extraction refluxing orthogonal test |
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