上调血红素氧合酶-1对实验性糖尿病大鼠血管内皮功能及血压的影响

 目的 探讨应用氯化亚锡(SnCl2)上调血红素氧合酶-l(HO-1)对实验性糖尿病大鼠血管内皮功能及血压的影响及其机制.方法 40只Wistar-Kyoto(WKY)大鼠分为4组,分别为WKY组、糖尿病大鼠(DWKY)组、WKY+SnCl2组及DWKY+SnCl2组.通过注射链佐霉素(STZ)制造DWKY模型共20只,其中10只进一步制作为DWKY+SnCl2组.WKY组共20只,其中10只进一步制作为WKY+SnCl2组.分别测量各组大鼠尾动脉血压,采用免疫印迹法测定股动脉HO-1、HO-2、凋亡相关蛋白(Bcl-2)表达量,HO活性,环氧化酶2(COX-2)水平,脂联素、肿瘤坏死因子-α(TNF-α)、超氧阴离子(O-2)水平,股动脉血管内皮反应性以及循环内皮细胞计数(CEC).结果 DWKY组中股动脉血管内皮反应性显著低于WKY组.SnCl2显著上调HO-1及Bcl-2表达,降低COX-2水平,增加HO活性[0.25±0.05 nmol胆红素/(mg·h)与0.17±0.025 nmol胆红素/(mg·h),P＜0.05],降低0-2、TNF-α水平,增加脂联素水平及股动脉血管内皮反应性,显著减少内皮细胞脱落并显著降低DWKY血压水平(收缩压100±1 mmHg与81±2 mmHg,P＜0.05).结论 SnCl2通过上调HO-1的表达以及增加HO活性,升高脂联素水平,同时改善血管内皮功能,从而降低糖尿病大鼠的血压水平.

Effect of up-regulated heme-oxygenase-1 level on the vascular endothelial function and blood pressure in experimental diabetic rats

Objective To investigate the effect of up-regulated heme-oxygenase-1(HO-1 ) levels on the vascular endothelial function and blood pressure in experimental diabetes mellitus.Methods 40 Wistar-Kyoto rats(WKY) were divided into 4 groups:diabetic WKY(DWKY) group,DWKY + stannous chloride(SnCl_2 ) group,WKY group and WKY + SnCl_2 group.20 DWKY models were established through injections of streptozotocin(STZ),including 10 for the DWKY + SnCl_2 group.There were 20 rats in WKY group,including 10 WKY + SnCl_2 rats.Th...