| 高效液相色谱法测定心脉神胶囊中大黄素含量 |
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| 引用本文： | 曹健,黄林清,王芳,朱丽莎,张恩娟.高效液相色谱法测定心脉神胶囊中大黄素含量[J].中国药业,2010,19(1):26-27. |
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| 作者姓名： | 曹健 黄林清 王芳 朱丽莎 张恩娟 |
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| 作者单位： | 1. 第三军医大学新桥医院药学部,重庆,400037
2. 重庆医科大学药学系,重庆,400016 |
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| 摘 要： | 目的建立测定心脉神胶囊中大黄素含量的高效液相色谱（HPLC）法。方法色谱柱为Hypersil ODS柱（150mm×4．6mm，10μm），流动相为甲醇-0．02mol／L磷酸（75：25），检测波长438nm，流速1．2mL／min。结果大黄素质量浓度在0．0022～0．0110g／L范围内与峰面积值呈良好的线性关系，线性回归方程为A=33111090．9C＋396．4，r=0．9998，平均回收率为100．35％，RSD为0．96％。结论HPLC法适用于心脉神胶囊中大黄素的含量测定。
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| 关 键 词： | 高效液相色谱法 心脉神胶囊 大黄素 |
Assaying of Emodin in Xinmaishen Capsule by HPLC |
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| Cao Jian,Huang Linqing,Wang Fang,Zhu Lisha,Zhang Enjuan.Assaying of Emodin in Xinmaishen Capsule by HPLC[J].China Pharmaceuticals,2010,19(1):26-27. |
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| Authors: | Cao Jian Huang Linqing Wang Fang Zhu Lisha Zhang Enjuan |
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| Institution: | 1. Xinqiao Hospital, Third Military Medical University, Chongqing, China 400037; 2. Department of Pharmacy, Chongqing Medical University, Chongqing, China 400016) |
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| Abstract: | Objectlve To establish a HPLC method for the assaying of emodin in Xinmaishen Capsule. Methods The assaying was performed on the Hypersil ODS column（150 mm × 4.6 mm, 10 μm）. The mobile phase consisted of methanol -0. 02 mol/L phosphoric acid（75 ： 25） with the flow rate of 1.2 mL/min and the detective wavelength at 438 nm. Results The linear sample size range for emodin was 0. 002 2- 0. 011 0 g/L, the equation was A = 33 111 090. 9 C ＋ 396.4（ r = 0. 999 8）. The average recovery was 100.35% （ RSD = 0. 96% ）. Conclusion The method is suitable for the quality control of emodin in Xinmaishen Capsule. |
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| Keywords: | HPLC Xinmaishen Capsule emodin |
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