肺缺血再灌注中内皮细胞功能的研究

目的 探讨肺缺血再灌注（I－R）中内皮细胞功能的变化。方法 制备兔肺缺血再灌注模型，设立伪手术对照，测定缺血期和缺血后再灌注不同时相的血浆一氧化氮（NO）和内皮素（ET－1)的水平，分析NO，ET－1与血气指标、肺水肿等指标的关系。结果 缺血再灌注组与对照组比较，血浆NO，ET－1水平显著增高。NO／ET－1值明显降低，动脉氧分压明显降低，肺湿／干重比增加。ET－1与动脉氧分压呈负相关，与肺湿／干重比呈正相关。结论 肺I－R状态下，内皮细胞功能紊乱与低氧和肺水肿有关联。

The function of endothelium in lung with ischemia reperfusion

Objective To study the function of circulating endothelium in lung with ischemia reperfusion. Methods The rabbit's pulmonary models of ischemia reperfusion (I- R) injury were established. 14 rabbits were randomized into sham treated control and I-R ( n = 7) groups. Blood specimens were collected in different testing periods in control and I-R groups to detect the concentrations of plasma NO and ET - 1 and arterial oxygen tension. Wet and dry weight were calculated. Results Compared with those in sham treated control groups, the plasma concentrations of NO and ET - 1 in I- R animals were increased significantly. The relationship analysis showed that the concentrations of ET- 1 had signifi-cantly negative relation with that of Pa02 and positive relation with the value of the wet: dry weight ratios. Conclusion In the I-R group, the function of circulation endothelium had been damaged which may be related to hypoxia and pulmonary edema.