非小细胞肺癌中3p^25杂合性丢失研究

目的为探讨非小细胞肺癌（ＮＳＣＬＣ）细胞中第三号染色体短臂上３ｐ２５位点上等位基因的杂合性丢失（ＬｏｓｓｏｆＨｅｔｅｒｏｚｙｇｏｓｉｔｙＬＯＨ）与肺癌发生发展的相关性。方法采用银染聚合酶链反应（ＰＣＲ）结合二核苷酸（ＣＡ）ｎ重复程序出现多态性评价杂合性丢失方法，分析５８例ＮＳＣＬＣ组织中３ｐ２５等位基因的杂合性丢失。结果５８例肺癌组织中有２９例出现３ｐ２５ＬＯＨ，总的杂合性丢失率为５０％。３ｐ２５的杂合性丢失率在１８例腺癌中为１２例（６６．７％），明显高于鳞癌（４２．９％，１５／３５），有显著性统计学意义（Ｐ＜０．０５），但３ｐ２５杂合性丢失与ＮＳＣＬＣ临床分期关系不明显，Ⅰ期为４２．１％，Ⅱ期为５５．６％和Ⅲ期为５２．２％，５例正常胎儿肺组织均不出现３ｐ２５ＬＯＨ。结论３ｐ２５杂合性丢失在ＮＳＣＬＣ中普遍存在，从而说明３ｐ２５位点处存在着某些与肺癌发生发展有关的抑癌基因。

DETECTION OF ALLELE SPECIFIC CHROMO SOME 3p 25 DELETION BY PCR LOH ANALYSIS IN NON SMALL CELL LUNG CANCER

bjective:Relationship between deletion of a wild type allele on chromosome 3p 14-25 and non small cell lung cancer(NSCLC)was studied.Methods:The 3p 25 loss of heterozygosity(LOH)was analysed in patients with NSCLC by polymerase chain reaction based assay for dinucleatied repeat polymorphisms.Results:Twenty nine(50%)out of 58 cases of NSCLC were found to have the LOH,but none in 5 embryonic lung tissues.This LOH frequency was 66 7% in adeno carcinoma and 42 9% in squamous cell carcinoma with significant difference between them ( P <0 05).The LOH frequency was not correlated with clinical TNM stages of NSCLC( P >0 05).Conclusions:Deletion of the wild type allele 3p 25 appeared to be a common structural alteration occurred in various types of lung cancer,and was an early molecular event involved in lung carcinogenesis.