结直肠癌术后吻合口瘘与表皮生长因子、转化生长因子-β1、血管内皮生长因子基因多态性的关系

目的 观察表皮生长因子(EGF)61*G/A、转化生长因子-β1(TGF-β1)-509*T/C、血管内皮生长因子(VEGF)936*T/C基因功能多态性对术后结肠吻合口瘘的影响.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测伴有和不伴有术后结肠吻合口瘘结直肠癌患者的EGF 61*G/A,TGF-β1-509*T/C,VEGF 936*T/C基因型.结果 伴有术后结肠吻合口瘘的结直肠癌患者TGF-β1-509*T/T基因型比例(0.0%)低于无术后吻合口瘘的患者(13.2%),两者差异有统计学意义(P＜0.05).伴有术后结肠吻合口瘘的VEGF 936*C/C基因型和C等位基因比例(63.9%和80.6%)低于无术后吻合口瘘的患者(82.6%和90.5%),两者差异有统计学意义(基因型χ2=5.753,P＜0.05;等位基χ2=5.275,P＜0.05).伴有术后结肠吻合口瘘的结直肠癌患者EGF 61*G/G基因型比例(25.0%)低于无术后吻合口瘘的患者(35.5%),但两者差异无统计学意义(χ2=1.391,P＞0.05).结论 TGF-β1-509*T/T基因型,VEGF 936*C/C基因型或936*C等位基因有助于减少术后结肠吻合口瘘的发生.

Association between anastomotic leakage after colorectal cancer surgery and functional polymorphism in epidermal growth factor, transforming growth factor-β1,vascular endothelial growth factor gene

Objective To understand the effect of EGF,TGF-β1,VEGF on the anastomotic leak age after colorectal cancer surgery by the investigation of EGF 61*G/A,TGF-β1-509*T/C,VEGF 936 *T/C gene functional polymorphism.Methods PCR-RFLP was employed to study the EGF 61*G*A, TGF-β1-509*T/C,VEGF 936*T/C genotypes in colorectal cancer patients with or without anastomotic leakage after surgery.Results The frequency of TGF-β1-509*T/T genotype in colorectal cancer pa tients with anastomotic leakage(0.0)was significantly lower than that in the patients without anastomotic leakage(13.2％)(P＜0.05).The frequency of VEGF 936*C/C genotype and C allele in colorectal cancer patients with anastomotic leakage(63.9％and 80.6％respectively)was significantly lower than in the patients without anastomotic leakage(82.6％and 90.5％respectively)(genotype χ2=5.753,P＜ 0.05；allele χ2=5.275,P＜0.05).Although the EGF 61*G/G genotype in colorectal cancer patients with anastomotic leakage(25.0％)was less frequently found than in the patients without anastomotic leakage(35.5％),there was no statistically significant difference(χ2=1.391,P＞0.05).Conclusion TGF-β1-509*T/T genotype,VEGF 936*C/C genotype and C allele decrease the risk of anastomotic leakage after surgery for colorectal cancer.