| 乙型肝炎病毒C基因启动子变异株克隆的构建及应用 |
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| 引用本文： | 陆建荣. 乙型肝炎病毒C基因启动子变异株克隆的构建及应用[J]. 齐齐哈尔医学院学报, 2002, 23(3): 246-247 |
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| 作者姓名： | 陆建荣 |
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| 作者单位： | 江苏省南通市第三人民医院病毒性肝炎研究室,226006 |
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| 摘 要： | 目的：构建乙型肝炎病毒（HBV）C基因启动子（BCP）变异株克隆。方法：采用PCR产物克隆法。结果：构建成功pBCM质粒，经克隆鉴定及错配PCR－RFLP证实为目的克隆。结论：质粒pBCM可作为错配PCR－RFLP检测HBV BCP基因变异株检测的阳性对照。
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| 关 键 词： | 乙型肝炎病毒 C基因启动子 变异 克隆 |
Construction of HBV Basic Core Promoter Mutants |
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| Lu Jianrong.. Construction of HBV Basic Core Promoter Mutants[J]. Journal of Qiqihar Medical College, 2002, 23(3): 246-247 |
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| Authors: | Lu Jianrong. |
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| Abstract: | Objective To construct hepatitis B virus(HBV) basic core promoter(BCP) mutants. Methods Mismatched primer polymerase chain reaction products were digested and cloned into pUC18.Results After clone identification and mismatched primer polymerase chain reaction-restriction fragment length polymorphism(mpPCR-RFLP) analysis, it was proved to be the HBV BCP mutant clone. Conclusions pBCM can be used as the standard strain to control the detection of HBV BCP mutants. |
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| Keywords: | HBV BCP Mutant Clone |
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