9401对H22肝癌小鼠放射增敏效应的研究

目的 探讨9401对H22肝癌小鼠的放射增敏作用。 方法 建立H22肝癌小鼠模型，按照给药和照射的不同，分为空白对照组、单纯照射组、烟酰胺联合照射组、9401高剂量联合照射组、9401中剂量联合照射组和9401低剂量联合照射组。照射后观察H22肝癌小鼠的肿瘤生长情况、记录肿瘤照射后的生长时间，并计算肿瘤生长的延迟时间和各组的放射增敏系数。照射后28 d处死小鼠，剥瘤称重，计算抑瘤率。 结果 9401高、中、低各剂量联合照射组均能延缓肿瘤生长，其中9401高、中剂量联合照射组与烟酰胺联合照射组相比，差异有统计学意义（t=24.7和7.5，P均＜0.01），且9401高、中剂量组辐射敏感性均显著增高，增敏系数分别为2.13、1.73，明显高于烟酰胺联合照射组的增敏系数1.61，差异有统计学意义（t=2.26和9.04，P均＜0.05）；9401高、中、低各剂量联合照射组的抑瘤率分别为64.5%、50.9%、42.6%，烟酰胺联合照射组的抑瘤率为53.2%，9401高剂量组抑瘤效果显著高于烟酰胺联合照射组，差异有统计学意义（t=2.8，P < 0.05）。9401高、中、低各剂量组与单纯照射组相比，抑瘤率差异有统计学意义（t=5.7，4.0和2.2，P均＜0.05）。 结论 9401对H22肝癌小鼠肿瘤生长有明显的抑制作用，抑制肿瘤作用随给药剂量的增加而逐步增强，放射增敏效果显著，临床应用前景广阔。

Radiosensitizing effects of 9401 on mice bearing H22 hepatoma

Objective To investigate the radiosensitizing effects of 9401 on mice bearing H22 hepatoma. Methods Mouse model bearing H22 hepatoma cells were established. Mice were randomly divided into six groups, the control group, the radiation group and four treatment groups including 9401 at high, medium and low dosages and nicotinamide combined with radiation. After irradiated, the growth of tumor was observed, the time of tumor growth was recorded, the delay time of tumor growth and enhancement factor(EF)were calculated. After 28 days, the mice were killed, the tumors were stripped and inhibition rate was calculated. Results Groups of 9401 combined with radiation could pestpone tumor growth. The difference was statistically significant between 9401 groups at high, medium dosages combined with radiation and nicotinamide combined with radiation group (t=24.7 and 7.5, both P < 0.01). Compared with radiation alone group, groups of 9401 combined with radiation had significant radiosensitizing effect. The enhancement factor of 9401 combined with radiation groups at high and medium dosages were 2.13 and 1.73 respectively, they were significant higher than nicotinamide combined with radiation group(t=2.26 and 9.04, both P < 0.05). The inhibition rate of 9401 groups at high, medium and low dosages combined with radiation were 64.5%, 50.9% and 42.6% respectively. The inhibition rate of nicotinamide group combined radiation was 53.2%. The inhibition rate of 9401 at high dosage combined with radiation had significant difference with nicotinamide combined radiation(t=2.8, P < 0.05). Nicotinamide combined with radiation group, 9401 combined with radiation groups could significant inhibit the growth of tumors compared with radiation alone group(t=5.7, 4.0 and 2.2, all P < 0.05). Conclusion 9401 can inhibit the tumor growth and the inhibition effect increases gradually with the drug dose increasing. It also has radiosensitizing effects on mice bearing H22 hepatoma and present broadly clinical practice prospect.