| 两种ELISA方法检测日本血吸虫病循环抗原的比较 |
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| 引用本文： | 裘丽妹,张永红,李浩,薛海筹. 两种ELISA方法检测日本血吸虫病循环抗原的比较[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(4): 227-228 |
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| 作者姓名： | 裘丽妹 张永红 李浩 薛海筹 |
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| 作者单位： | 中国预防医学科学院寄生虫病研究所**,上海 200025 |
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| 基金项目： | “九五”科技攻关资助项目(96-906-04-04)世界卫生组织疟疾、血吸虫病和丝虫病合作中心;卫生部寄生虫病原与媒介生物学重点实验室 |
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| 摘 要： | [目的 ]比较两种ELISA方法检测血吸虫病患者血清中的循环抗原的效果。 [方法 ]以单克隆抗体为基础的斑点 ELISA和夹心 ELISA。 [结果和结论 ]斑点 ELISA与夹心 ELISA的阳性检出率分别为 48 0 %～ 98 8%和 12 0 %～ 93 8%。两法的特异性依次为 95 4%～ 10 0 %及 90 %。
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| 关 键 词： | 日本血吸虫 循环抗原 斑点ELISA 夹心ELISA |
| 文章编号： | 1000-7423(2000)-04-0227-02 |
| 修稿时间： | 1999-04-06 |
COMPARISON OF CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS JAPONICA PATIENTS USING TWO METHODS OF ELISA |
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| QIU Li-zhu ZHANG Yong-hong LI Hao XUE Hai-chou. COMPARISON OF CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS JAPONICA PATIENTS USING TWO METHODS OF ELISA[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2000, 18(4): 227-228 |
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| Authors: | QIU Li-zhu ZHANG Yong-hong LI Hao XUE Hai-chou |
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| Affiliation: | Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025. |
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| Abstract: | OBJECTIVE: To compare 2 kinds of ELISA methods for detecting circulating antigen in schistosomiasis patients. METHODS: Monoclonal antibody-based dot-ELISA and sandwich ELISA. RESULTS AND CONCLUSION: The positive rates of dot-ELISA were higher than or similar to those of sandwich ELISA, the former being 48.0%-98.8% and the latter being 12.0%-93.8%. The specificities were 95.4%-100% and 90.0%, respectively. |
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| Keywords: | schistosomia japonicum circulating antigen dot-ELISA sandwich-ELISA |
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