温阳益气、活血利水法对冠脉结扎心肌梗死后心衰大鼠NT-proBNP的影响

目的 观察温阳益气、活血利水法对心肌梗死后心衰大鼠N-末端脑钠肽前体(NT-proBNP)的影响.方法 结扎大鼠冠状动脉左前降支,制作大鼠心肌梗死后心衰模型,分为对照组,假手术组,模型组,温阳益气、活血利水法组方高、中、低剂量组,卡托普利组.中药干预后,检测大鼠血清NT-proBNP水平.结果 各模型组血清NT-proBNP水平均显著高于假手术组(P＜0.01);与模型组比较,温阳益气、活血利水法组方各剂量组血清NT-proBNP水平均降低(P＜0.05或P＜0.01);卡托普利组血清NT-proBNP水平与温阳益气、活血利水法组方高剂量组相近(P＞0.05);空白对照组和假手术组比较,血清NT-proBNP水平无显著性差异.结论 温阳益气、活血利水法可降低心肌梗死后心衰大鼠血清NT-proBNP水平.

The Influence of Yang Warming and Qi Benefiting,Blood Activating and Water Disinhibiting Therapy on NT-proBNP in Heart Failure Rats after Myocardial Infarction

Objective To investigate the effects of yang warming and qi benefiting,blood activating and water disinhibiting therapy(YQBW)on serum amino terminal pro-brain natriuretic peptide(NT-proBNP) level in the heart failure rats after myocardial infarction.Methods Rat models with post-myocardial infarction heart failure were established by left anterior descending coronary artery ligation.The rats were divided into seven groups:Control group,sham group,model group,high,medium,low-dosage of YQBW groups,and captopril group.After intervention,the serum NT-proBNP levels were assayed by electrochemiluminescence double antibody sandwich immunoassay.Results The levels of serum NT-proBNP in models group were significantly higher than that in the sham group(P<0.05).Compared with model group,the NT-proBNP level was decreased with the increase of the dosage in YQBW groups(P<0.05).In both high-dosage YQBW group and captopril group,the NT-proBNP level was closely same(P>0.05).There was no significantly difference of serum NT-proBNP level in control group and sham group(P>0.05).Conclusion Yang warming and qi benefiting,blood-activating and water-disinhibiting therapy could decrease the serum NT-proBNP levels in the heart failure rats after myocardial infarction.