通心络预给药2h对猪急性心肌梗死再灌注后心肌无再流和细胞因子变化的影响

目的 评价急性心肌梗死（AMI）猪缺血再灌注前2 h给予通心络对血清细胞因子水平和心肌无再流变化的干预效果。

Effect of Tongxinluo on Mini-swine Cytokines and Myocardial No-reflow in Early Reperfusion of Acute Myocardial Infarction

Objective To assess the effect of Tongxinluo on cytokines and myocardial no-reflow in early reperfusion of acute myocardial infarction（AMI）.Methods Forty mini-swine were divided into five groups randomly,sham group,control group,low dose（0.1 g/kg）,medium dose（0.2 g/kg） and high dose（0.4 g/kg） group of Tongxinluo（which were administered 2 h before reperfusion）,eight swine in each group.Animals except those in the sham group were subjected to 1.5 h of coronary occlusion followed by 3 h of reperfusion.Serum contents of P-selectin,intercellular adhesion molecule 1（ICAM-1）,vascular cell adhesion molecule 1（VCAM-1）,interleukin 6（IL-6） and interleukin 10（IL-10）,as well as myocardial contrast echocardiography（MCE） were evaluated at baseline,and after 1.5 h of AMI and 3 h of reperfusion.Results（1） Compared with that of the control group,high dose of Tongxinluo could reduce serum contents of P-selectin and ICAM-1 at 1.5 h of AMI（all P〈0.05）,and P-selectin,ICAM-1,VCAM-1,and IL-6 at 3 h of reperfusion significantly（all P〈0.05）,accompanied by significantly elevated IL-10（P〈0.05）.（2） Compared with that of control group,high dose of Tongxinluo could reduce no-reflow area at 3 h of reperfusion significantly [（6.59±1.73）cm2 vs（4.68±1.53）cm2,P〈0.05].Conclusion High dose of Tongxinluo could effectively reduce serum contents of adhesion and pro-inflammatory cytokines,regulate anti-inflammatory factor levels,and attenuate no-reflow area in the early reperfusion of AMI.It thus provided experimental basis for its clinical application.