Glucoresponsive neurons in rat ventromedial hypothalamic tissue slices in vitro |
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| Authors: | Taketoshi Ono Hitoo Nishino Masaji Fukuda Kazuo Sasaki Ken-Ichiro Muramoto Yutaka Oomura |
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| Affiliation: | 1. M.R.C. Neurochemical Pharmacology Unit, M.R.C. Centre, Medical School, Hills Road, Cambridge, U.K.;2. Department of Clinical Chemistry, Rigshospitalet, DK-2100 Copenhagen, Denmark |
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| Abstract: | Extracellular action potentials were recorded from rat ventromedial hypothalamic nucleus (VMH) tissue slices in vitro. The identified glucoresponsive and non-glucoresponsive neurons were then visually located and observed by intracellular horseradish peroxidase staining. Glucoresponsive neurons, which were predominantly multipolar, were found near the center of the VMH, while non-glucoresponsive neurons, half of which were bipolar and half multipolar, were found randomly throughout the VMH. |
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| Keywords: | ventromedial hypothalamus glucoreceptor neuron brain slice tissue slice horseradish peroxidase |
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