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冬虫夏草及其虫草菌粉中核苷类成分的HPLC测定
引用本文:陈博 金瓯. 冬虫夏草及其虫草菌粉中核苷类成分的HPLC测定[J]. 中国生化药物杂志, 1998, 19(2): 88-90
作者姓名:陈博 金瓯
作者单位:浙江省药品检验所!杭州,310004
摘    要:采用反相高效液相色谱法、SpherisorbC18柱,甲醇-pH4.0磷酸盐缓冲液(10∶90)为流动相,检测波长258um,测定冬虫夏草、发酵虫草菌粉与虫草头孢菌粉中尿苷、鸟苷及腺苷的含量。平均回收率为96.1%~99.1%;此方法简便、准确、重现性好。

关 键 词:冬虫夏草  尿苷  鸟苷  腺苷  高效液相色谱

Analysis of Nucleosines in the Cordyceps Sinensis and Its Fermental Powder by RP-HPLC
Chen Bo, Jin Ou. Analysis of Nucleosines in the Cordyceps Sinensis and Its Fermental Powder by RP-HPLC[J]. Chinese Journal of Biochemical Pharmaceutics, 1998, 19(2): 88-90
Authors:Chen Bo   Jin Ou
Affiliation:Chen Bo; Jin Ou (Zhejiang Provincial Institute for drug Control (Hangzhou 310004
Abstract:A method for the determination of Uridine, Guanosine and Adenosine in the Cordyceps sinesis and its two fermental powders by RP-HPLC was described. On Spherisorb C18 column using methnolpH4. 0 phosphate (10: 90) as mobile phase and detected at 258 nm, the standards and samples could be separated satisfactorily. The average recovery of three nucleosines were ftom 96.1 %to 99. 1%.The total contents of three nucleosines in the fermental Powder A and Powder B were 8. 72 mg/g and l6. o9 mg/ g, resPectively. The result showed that the method was simple, accurate and more reproducible.
Keywords:Cordyceps sinensis   Uridine  Guanosine   Adenosine   HPLC
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