EGFR抑制剂C225对人前列腺癌细胞的放射增敏作用

目的 探讨表皮生长因子抑制剂C225对人前列腺癌细胞株(DU145) 放射敏感性的影响。方法 实验组用表皮生长因子抑制剂C225(100 nmol/L)处理后,⁶⁰Co γ射线(吸收剂量率1.953 Gy/min)对体外培养的DU145细胞进行0、2、4、6 和8 Gy 照射,用MTT法、集落形成法检测细胞增殖和细胞存活率;用单击多靶模型拟合剂量存活曲线;用流式细胞仪检测细胞周期和凋亡。结果 C225对照射后DU145细胞增殖有明显抑制作用。C225组的放射生物学参数值(D₀、D_q、N、SF₂)较对照组低。C225组和对照组的RBE 比值为1.39。C225组处于S期的细胞比例降低,出现明显的G₀/G₁期阻滞。C225组细胞的早期凋亡率在照射剂量达4 Gy后明显高于对照组(t=-14.55,P＜0.05)。结论 表皮生长因子抑制剂C225通过抑制细胞增殖、G₀/G₁ 期阻滞和诱导凋亡来增加人前列腺癌细胞放射敏感性。

Radiosensitization effect of EGFR inhibitor C225 on prostate cancer cell

Objective To determine the effects of EGFR inhibitor (3225 on radiosensitivity of human prostate cancer cells. Methods Human prostate cancer DU145 cells were irradiated with 60Co ( 1.953 Gy/min) at various doses of 0,2,4,6,8 Gy in the presence or absence of C225 (100 nmol/L). The cellular proliferation and cell surviving rate were evaluated by MTT and the colony-forming assay. The cell cycle distribution and cell apaptosis were tested by flow cytometry. Results C225 caused significant anti-proliferative effect on irradiated DU145 cells. The radiobiological parameters of C225 group, including D0, Dq, N, SF2, were lower than those of control group. The RBE for C225 group compared with control group is 1.39. Cells treated with C225 showed significant G0/G1 phase arrest and a decrease in S phase over the dose of 4 Gy in comparison with the control. Higher apaptosis rate over 4 Gy was observed in C225-treated cells compared to the control (t=-14.55, P< 0.05). Conclusions EGFR inhibitor C225 could increase the radiosensitivity of DU145 cells through anti-proliferation, G0/G1 phase arrest and apoptosis induction.