冠心病患者血小板α膜糖蛋白的表达

目的 检测冠心病患者全血血小板α膜糖蛋白(CD62P)阳性表达与冠状动脉病变程度,探讨CD62P在冠心病中的作用.方法 采用流式细胞术(FCM)测定70例冠心病患者的外周血CD62P的阳性表达水平,其中稳定型心绞痛(SAP组)28例、不稳定型心绞痛(UAP组)26例和急性心肌梗死(AMI组)16例,20例非冠心病患者作为对照.结果 70例冠状动脉造影均为阳性,CD62P表达率在不稳定型心绞痛组为(28.3±20.1)%,急性心肌梗死组为(20.5±16.3)%,稳定型心绞痛组为(12.8±13.3)%,非冠心病对照组为(9.9±6.4)%,不稳定型心绞痛组与对照组及稳定型心绞痛组比较、冠脉1～3支病变比较差异均有统计学意义.结论 不稳定型心绞痛者体内CD62P水平明显增高,与冠脉病变的严重程度相关.

Level of alpha-granule membrane protein (CD62P) in coronary heart disease

OBJECTIVE: To investigate the role of alpha-granule membrane protein (CD62P), marker of plate activation, in the patients with coronary heart disease (CHD) and to investigate the relationship between the severity of coronary artery stenosis and platelet activation. METHODS: Peripheral blood samples were collected from 70 patients with CHD, including 28 cases of stable angina pectoris (SAP), 26 cases of unstable angina pectoris (UAP), and 16 cases of acute myocardial infarction (AMI), all of which underwent coronary arteriography, and 20 angiographically normal persons as no-CHD controls. The CD62P level was analyzed by flow cytometry (FCM). RESULTS: Coronary arteriography showed positivity in 70 of the 90 CHD patients. The CD62P positive rate of the UAP group was (28.3 +/- 20.1)%, significantly higher than those of the SAP group (12.8 +/- 13.3)%] and no-CHD group (9.9 +/- 6.4)%, both P < 0.05]. The CD62P positive rate of the double vessel disease group was (24.0 +/- 17.8)%, not significantly different from that of the three-vessel disease group (22.6 +/- 20.4)%], but significantly higher than that of the single-vessel disease group (11.1 +/- 8.5)%] and that of the no-CHD group (9.9 +/- 6.4)%, both P < 0.05]. CONCLUSION: The platelet alpha-granule membrane protein CD62P level is higher in the UAP patients. It is related to the pathophysiological process of UAP and the severity of coronary artery stenosis.