慢性不可预见性温和应激后大鼠行为及海马细胞支架的改变

目的研究慢性不可预见性温和应激所致的动物行为学改变及细胞支架微管系统的动态性改变。方法将大鼠随机分为应激模型组（8只，以下简称模型组）和对照组（8只），对模型组大鼠进行连续21d的慢性不可预见性应激。进行行为学观察，使用western blotting检测乙酰化微管蛋白（Acet—Tub），酪氨酸微管蛋白（Tyr—Tub）。结果（1）模型组大鼠慢性应激后糖水偏好及自主活动显著减低，与对照组有显著差异；（2）模型组大鼠慢性应激后海马Acet—Tub表达升高，Tyr—Tub表达减低，与对照组有显著差异。结论慢性应激后微管动态性减低，神经可塑性受损。

Behavioral and hippocampal cytoskeletal alterations in rats following chronic unpredictable mild stress

Objective To investigate behavior and hippocampal cytoskeletal alterations induced by chronic and unpredictable mild stress. Methods Sixteen rats were randomly divided into two groups, with 8 exposed to 21 consecutive days of chronic unpredicted mild stresses （CUMS） as depression models, and 8 as normal controls. Behavioral changes in these rats were analyzed in an Open--Field paradigm, and compared to a control group of normal rats. The expression of hippocampal cytoskeletal microtubulin was analyzed using Western Blot. Results （1） Compared with the normal rats, sucrose preference, total traveling distance, velocity and frequencies of rearing were significantly reduced in the de- pressed rats （P 〈 0.01） ; （2） The Acet--Tub expression was remarkably elevated in CUMS rats compared to control rats, while a significant reduction in Tyr--Tub expression was displayed in CUMS rats （P 〈 0.01）. Conclusions These findings provide evidence that rats exposed to stress showed impairment of cytoskeletal microtubular dynamic and structural neuronal plasticity, providing insight into the role of cytoskeleton in the depression.