白藜芦醇诱导KG-1细胞凋亡作用与bcl-2／bax表达相关性研究

本研究观察白藜芦醇（RES）体外诱导KG-1细胞凋亡的作用，探讨白藜芦醇诱导白血病细胞凋亡的可能作用机制。以不同浓度的白藜芦醇作用于KG-1细胞，用噻唑蓝（MTT）比色法分析细胞生长状态；透射电镜观察KG-1细胞凋亡的形态学变化；流式细胞术（FCM）测定细胞凋亡与周期分布；半定量RT-PCR、流式细胞技术检测细胞bel-2、bax表达水平。结果表明：白藜芦醇可明显抑制KG-1细胞增殖（P〈0．05），与对照组比较，白藜芦醇作用后KG-1细胞发生S期阻滞（P〈0．01），细胞凋亡增高（P〈0．01），bcl-2表达下调（P〈0．05），而bax表达明显上调（p〈0．01）。结论：白藜芦醇可诱导KG-1细胞凋亡，其机制可能与下调bcl-2、上调bax表达水平有关。

Relationship between Apoptotic Effect of Resveratrol on KG-1 Cells and Expression of bcl-2/bax

This study was aimed to explore the apoptotic effect of Resveratrol(RES) on KG-1 cells and the expression of bcl-2/bax in vitro,and to clarify the possible mechanism of apoptotic effect of RES on leukemia cells.After treating with different concentrations of RES,the suppressive effect of RES on proliferation of KG-1 cells was analyzed by MTT method.Transmission electron microscope technique were used to detect the apoptosis status of KG-1 cells.The cell cycle and apoptosis precentage of KG-1 cells treated with RES were detected by flow cytometry.The expressions of bcl-2,bax mRNA and protein were assessed by semiquantitative RT-PCR and flow cytometry.The results showed that RES could obviously inhibit proliferation of KG-1 cells(p<0.05).Compared with the control group,the cell number in S phase of KG-1 cells treated with RES increased(p<0.05),the apoptosis rate enhanced significantly(p<0.01)and the expression level was down-regulated,while expression level of bax was obviously up-regulated(p<0.01).It is concluded that RES significantly induces apoptosis of KG-1 cells in vitro,which is probably related to the down-regulation of bcl-2 expression and up-regulation of bax expression.