全自动微生物鉴定药敏分析仪对临床相关细菌的鉴定能力及应用价值

目的:探讨Vitek 2 Compact型全自动微生物鉴定药敏分析仪对病原菌的鉴定能力及临床价值。方法:选取某医院各科患者的呼吸道、泌尿生殖道、脓液、血液等样本中分离的病原菌126株和本院实验室保存的参考菌株60株以及4种质控菌株作为本次检测的菌株。采用法国生物梅里埃公司生产的Vitek 2 Compact型全自动微生物鉴定药敏分析仪及相应的鉴定卡进行鉴定，分析鉴定结果正确率、鉴定时间及药敏结果。结果:4种质控菌株中每种菌株各测试10次，共40次，鉴定重复率为100%。90株革兰阴性菌中，鉴定正确的菌株为87株，鉴定正确率为96.67%；96株革兰阳性菌中，鉴定正确的菌株为93株，鉴定正确率为96.88%。肠杆菌属、非发酵糖菌和链球菌属、微球菌属4种菌株均在9 h以内完成鉴定，其中肠杆菌属和微球菌属在6 h内完成了85%左右的鉴定。革兰阴性菌的药敏结果符合率为93.94%，革兰阳性菌的药敏结果符合率为93.40%，总符合率为93.66%。结论:Vitek 2 Compact型全自动微生物鉴定药敏分析仪对病原菌的鉴定正确率较高，能快速给出鉴定结果，对指导疾病诊断和抗菌药物的合理使用具有重要的临床价值。

Identification capability and application value of automatic microorganism identification drug sensitivity analyzer for clinically relevant bacteria

Objective: To investigate the identification capability and application value of VITEK 2 system automatic microorganism identification drug sensitivity analyzer for clinical bacteria.Methods: One hundred and twenty-six strains of pathogenic bacteria isolated from respiratory tract,urogenital tract,pus and blood sample were selected,and 60 reference strains were served as control.The drug sensitivity of pathogenic bacteria was identified using VITEK 2 system automatic microorganism identification drug sensitivity analyzer,the correct rate,identification time and drug sensitivity of whose were analyzed.Results: The four kinds of germplasm control strains was tested for 10 times,a total of 40 times,the repetition rate of identification was 100%.Among 90 strains of gram negative bacteria,87 strains were identified,and the correct identification rate of which was 96.67%.Among 96 strains of gram positive bacteria,93 strains were identified,and the correct identification rate of which was 96.88%.The identifications of enterobacter sp,non fermentation bacteria,streptococcus sp.and micrococcus were completed within 9 h,the 85% identification of enterobacter sp.and micrococcus were completed within 6h.The coincidence rate of drug susceptibility in gram positive bacteria and gram negative bacteria were 93.94% and 93.40%,respectively,and the total coincidence rate was 93.66%.Conclusions: The correct identification rate of VITEK 2 automatic microorganism identification drug sensitivity analyzer is high,which can quickly identify and guide the rational use of antimicrobial drugs,and has important clinical value in disease diagnosis.