宫颈鳞癌中RECK与MMP-9的表达及意义

目的:探讨宫颈鳞癌中逆转录富含半胱氨酸蛋白（RECK）和基质金属蛋白酶（MMP）-9的表达及意义。方法:应用免疫组织化学法，检测20例正常子宫颈组织及70例子宫颈鳞状细胞癌中RECK和MMP-9的表达。结果:RECK蛋白在正常宫颈组织、子宫颈鳞癌组织中表达的阳性率分别为95.00%和31.43%，差异有统计学意义（P<0.01）；有淋巴转移组的阳性率较无淋巴转移组明显降低（P<0.01）。MMP-9在正常宫颈组织、子宫颈鳞癌组织中表达的阳性率分别为25.00%和80.00%，差异亦有统计学意义（P<0.01）；MMP-9表达在宫颈鳞癌有淋巴转移组的阳性率较无淋巴转移组明显升高（P<0.01）。RECK及MMP-9在宫颈鳞癌中的表达呈负相关关系（P<0.01）。结论:RECK在宫颈鳞癌中表达减少或缺失可能是通过上调MMP-9的表达从而促进宫颈鳞癌的侵袭与转移,RECK和MMP-9与宫颈鳞癌的浸润转移密切相关。

Expression and significance of RECK and MMP-9 in cervical squamous cell carcinoma

Objective:To investigate the expressions and significance of RECK and matrixmetalloproteinase-9(MMP-9)in the cervical squamous cell carcinoma. Methods:The expressions of RECK and MMP-9 in 20 cases of normal cervical tissue and 70 cases of cervical squamous cell carcinoma were detected using immune histochemical staining method. Results:The positive rates of RECK protein expression in normal cervical tissue and cervical squamous cell carcinoma tissue were 95. 00% and 31. 43%, respectively, the difference of which was statistically significant(P<0. 01). Compared with the cervical squamous cell carcinoma patients without lymph node metastasis,the positive rate of RECK in patients with lymph node metastasis significantly decreased(P<0. 01). The positive rates of MMP-9 protein expression in normal cervical tissue and cervical squamous cell carcinoma tissue were 25. 00% and 80. 00%, respectively,the difference of which was statistically significant(P <0. 01). Compared with the cervical squamous cell carcinoma patients without lymph node metastasis,the positive rate of MMP-9 in patients with lymph node metastasis significantly increased( P<0. 01). The expression of RECK in cervical squamous carcinoma was negative correlation with the expression of MMP-9(P<0. 01). Conclusions:The decreasing or absent of the RECK expression in cervical squamous carcinoma maybe though upregulate the expression of MMP-9 to promote the infiltration and metastasis of cervical squamous carcinoma. The RECK and MMP-9 are closely related to the infiltration and metastasis.