CD137表达与慢性乙型肝炎患者肝脏免疫病理损伤程度的相关性研究

目的:探讨CD137表达与慢性乙型肝炎（chronic hepatitis B,CHB）患者肝脏免疫病理损伤程度的相关性。方法:采用ELISA法检测受试者外周血中CD137L的水平；免疫荧光法检测肝组织中CD137的表达；Western blot法检测肝组织中CD137和CD28蛋白水平表达；荧光定量PCR检测肝组织中CD137和CD28mRNA的表达。结果:重度CHB组患者血清CD137L的表达水平均明显高于健康对照组、轻度和中度CHB组患者（P<0.01）。肝组织中CD28mRNA在中度CHB组和重度CHB组患者的表达水平均较健康对照组及轻度CHB组患者明显升高（P<0.01）。重度CHB组患者CD137mRNA表达水平均较健康对照组、轻度CHB组和中度CHB组患者显著升高（P<0.01）。肝组织中CD28蛋白在中度CHB组和重度CHB组患者的表达水平均较健康对照组及轻度CHB组患者明显升高（P<0.01）。重度CHB组患者CD137蛋白表达水平均较健康对照组、轻度CHB组和中度CHB组患者中CD137蛋白表达水平显著升高（P<0.01）。结论:CD137的活化参与CHB患者肝组织免疫病理损伤过程，与其损伤程度有相关性。

Relationship between the CD137 expression and immunopathological injury degree of liver in patients with chronic hepatitis B

Objective:To investigate the correlation of the expression of CD137 and immunopathological injury degree of liver in patients with chronic hepatitis B( CHB) . Methods:The levels of CD137 in peripheral blood and liver tissue were measured by ELISA and immunofluorescent assay,respectively. The levels of the protein and mRNA expressions of CD137 and CD28 in liver tissue were detected using Western blot and qPCR,respectively. Results:The level of serum CD137 in severe CHB group was significantly higher than that in healthy control group,mild and moderate CHB groups(P<0. 01). The expression levels of CD28 mRNA in liver tissue in moderate and severe CHB groups were higher than that in healthy control group and mild CHB group(P<0. 01). The expression level of CD137 mRNA in liver tissue in severe CHB group was significantly higher than that in healthy control group,mild and moderate CHB groups(P<0. 01). The expression levels of CD28 protein in liver tissue in moderate and severe CHB groups were higher than that in healthy control group and mild CHB group(P<0. 01). The expression level of CD137 protein in liver tissue in severe CHB group was higher than that in healthy control group,mild and moderate CHB groups(P<0. 01). Conclusions:The activation of CD137 involves in the immunopathological injury process of liver in CHB patients,and is related to the degree of liver.