姜黄素对肾脏缺血再灌注损伤大鼠模型保护作用的研究

目的:探讨姜黄素对大鼠肾脏缺血再灌注损伤的保护作用及机制。方法:将60只SD大鼠随机分为假手术组(A组)、缺血再灌注组(B组)、姜黄素+缺血再灌注组(C组)和姜黄素+缺血再灌注组+锌-原卟啉组(D组),各15只。C组和D组术前5 d腹腔注射姜黄素100 mg/kg,A、B组注射等量的0.9%氯化钠注射液。D组大鼠术前60 min腹腔注射锌-原卟啉20μmol/kg。手术游离并阻断双肾肾蒂血管,60 min后恢复灌注,分别于4 h、12 h、24 h取血检测血清肌酐(SCr)、尿素氮(BUN)和肿瘤坏死因子-α(TNF-α)、血红素氧合酶-1(HO-1)、细胞间黏附分子-1(ICAM-1)的表达,24 h切除肾组织检测肾组织髓过氧化物酶(MPO)活性的表达水平。结果:C组和D组血清SCr、BUN、TNF-α、ICAM-1及肾组织MPO水平均高于A组,但低于B组(P<0.05~P<0.01);HO-1的表达低于A组,高于B组(P<0.05~P<0.01)。D组TNF-α和ICAM-1表达水平在各时间点均高于C组(P<0.05~P<0.01),SCr、BUN及肾组织MPO的表达在12 h和24 h高于C组(P<0.05~P<0.01),在4 h时差异无统计学意义(P>0.05);HO-1在各时间点表达均低于后者(P<0.05~P<0.01)。结论:姜黄素对肾脏缺血再灌注损伤具有保护作用,能够有效减轻肾组织的炎性及氧化应激损害。HO-1可能参与姜黄素对肾脏的保护。

The protective effect of curcumin on the ischemia-reperfusion injury in rats

Objective:To investigate the effects and its mechanism of curcumin Cur on ischemia-reperfusion(I/R) injury in rats. Methods:Sixty rats were randomly divided into the sham operation group ( group A ) , I/R group ( group B ) , curcumin +I/R group (group C) and Cur+I/R+Zn-protoporphyrin group(group D)(15 rats each group). group C and D were intraperitoneally injected with 100 mg/kg of curcumin before 5 days of operation, and group C were intraperitoneally injected with 20 μmol/kg of Zn-protoporphyrin before 60 mins of operation. Group A and B were injected with equivalent 0. 9% of sodium chloride. Except for group A,the bilateral renal blood vessels in other 3 groups were blocked,and poured into after 60 mins. The levels of serum creatinine( SCr) , blood urea nitrogen(BUN),tumor necrosis factor-α(TNF-α),heme oxygenase-1(HO-1),intercellular adhesion molecule-1(ICAM-1) in 4 groups were measured after 4 h,12 h and 24 h of reperfusion. The kidney tissue was excised,and the levels of myeloperoxidase ( MPO) activity in excised kidney tissue was measured. Results:The expression levels of SCr,BUN,TNF-α,ICAM-1 and MPO in group C and group D were higher and lower than those in group A and group B,respectively(P<0. 05 to P<0. 01). The expression levels of HO-1 in group C and group D were lower and higher than that in group A and group B,respectively(P <0. 05 to P <0. 01). The expression levels of TNF-α and ICAM-1 at each time-points in group D were higher than that in group C(P<0. 05 to P<0. 01). The expression levels of SCr and BUN in serum,and MPO in kidney tissue in group D at 12 h and 24 h were higher than that in group C (P<0.05 to P<0. 01),the differences of whose at 4 h were not statistically significant(P >0. 05),the level of HO-1 at each time-points in group D was lower than that in group C(P<0. 05 to P<0. 01). Conclusions:Curcumin can protect the kidneys against I/R injury,and effectively relieve the inflammatory reaction and oxidative stress of kidney tissue. HO-1 may be involved in the renal protective mechanism of curcumin.