胆囊癌组织中蛋白赖氨酸乙酰化的变化及临床意义

目的:探讨蛋白赖氨酸乙酰化在胆囊癌组织中的变化情况。方法:采用Western 免疫印迹法，分别检测30例胆囊癌组织和20例慢性胆囊炎组织中蛋白赖氨酸乙酰化的变化。结果:与慢性胆囊炎组织相比，胆囊癌组织中的蛋白赖氨酸乙酰化显著增加（P < 0.01），乙酰化的显著增加主要发生在相对分子质量为10 000~36 000的蛋白。结论:利用赖氨酸乙酰化单克隆抗体特异性能够检测到胆囊组织蛋白的赖氨酸乙酰化，且相对分子质量为10 000~36 000蛋白的赖氨酸乙酰化增加显著。

The change of protein lysine acetylation in carcinoma tissue of the gallbladder and its clinical significance

Objective: To investigate the change of protein lysine acetylation in carcinoma tissue of the gallbladder and its clinical significance.Methods: Western blot analysis was used to determine the change of protein lysine acetylation in tissues of the gallbladders from 30 patients with carcinoma of the gallbladder and 20 patients with chronic cholecystitis.The quantitative results of some bands in western blot were analyzed.Results: The protein lysine acetylation was significantly increased in gallbladder cancer tissue,compared to the chronic cholecystitis.The quantitative analysis indicated that the significant increase of protein lysine acetylation took place in proteins with molecular weight (Mw) from 10 000 to 36 000.Conclusions: The protein lysine acetylation of gallbladder tissue could be examined by the highly sensitive and specific antibody against lysine acetylation.the lysine acetylation was evidently increased in gallbladder cancer protein,especially in a subset of proteins of Mw 10000 to 36 000.