周期蛋白D1在先天性与后天性胆脂瘤中的表达

目的通过研究周期蛋白D1(CyclinD1)在先天性胆脂瘤和后天性胆脂瘤的表达,探讨其表达差异的意义。方法选取我院就诊的60例中耳胆脂瘤患者,其中先天性胆脂瘤25例,后天性胆脂瘤35例;同时选取同期3例慢性中耳炎手术患者正常耳廓后皮肤组织作为对照组。采用免疫组化方法检测胆脂瘤组织中CyclinD1的表达。结果 CyclinD1免疫组化染色在先天性胆脂瘤和后天性胆脂瘤中均为阳性表达,但后天性胆脂瘤中阳性表达率高于先天性胆脂瘤,其差异具有统计学意义(P<0.01);CyclinD1在正常皮肤中不表达。结论 CyclinD1在后天性胆脂瘤上皮表达增强,可能是炎症刺激所致。

Expression of cyclinD1 in congenital and acquired cholesteatoma

Objective To study the expression of cyclinD1 in congenital and acquired cholesteatoma and its significance.Methods 25 samples of congenital cholesteatoma,35 of acquired cholesteatoma and 3 of normal auricle skin in the patients of chronic otitis media were included.The expression of cyclinD1 in these samples was detected with immunohistochemical method.Results CyclinD1 was positively expressed in cholesteatoma,but not in normal skin.As in the cholesteatoma,the positive rate of cyclinD1 in acquired ones was significantly higher than that in the congenital and the difference was statistically significant(P<0.01).Conclusion Acquired cholesteatoma has stronger proliferation ability than congenital cholesteatoma,which may be caused by inflammatory stimuli.