HPVL1蛋白和IL-2在宫颈病变中的表达及临床意义

目的探讨人乳头状瘤病毒(HPV)L1壳蛋白及IL-2在不同宫颈病变当中的表达情况及相关性。方法收集2011年4月-2011年9月在山西省肿瘤医院就诊的宫颈液基细胞学检查诊断≥未明确诊断意义的不典型鳞状细胞(ASCUS),且检测HPV DNA均为阳性,同时有组织病理学诊断(作为金标准)的标本159例:慢性宫颈炎48例,宫颈上皮内瘤变99例(CINⅠ级42例、CINⅡ27例、CINⅢ30例)以及浸润性鳞状细胞癌(SCC)12例,采用免疫组化方法检测HPV L1壳蛋白和IL-2在各级宫颈病变组织的表达情况。结果 159例患者中,HPV L1阳性表达为47.80%:在慢性宫颈炎中,HPV L1蛋白阳性表达为62.50%(30/48),CINⅠ为83.33%(35/42),CINⅡ为37.03%(10/27),CINⅢ为3.3%(1/30),SCC为0(0/12)。除了CINⅢ与SCC比较差异无统计学意义(P>0.05)外,其它不同宫颈病变间比较,差异有统计学意义(P<0.05);IL-2在各种宫颈疾病组织中阳性表达为64.78%:在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ及SCC组中的阳性分别为83.33%(40/48)、89.30%(37/42)、62.97%(17/27)、30%(9/30)和0(0/12)。IL-2在不同宫颈病变组之间的比较,差异均具有统计学意义(P<0.05)。结论 HPV L1壳蛋白与IL-2的阳性表达率均随着宫颈病变的演变及发展而呈降低趋势。二者在宫颈病变的发生发展中具有一定意义。子宫上皮内瘤变(CIN)生物学行为的预测对于癌前病变的筛查和预防有着具有重要的意义。

The expression and clinical significance of HPVL1 and IL-2 in cervical lesions

Objective To explore the expression and correlation of the human papilloma virus L1 capsid protein and IL-2 among the various cervical lesions.Methods Collected 159 cases in patients from April to September in 2011 in Shanxi Tumor Hospital for treatment of cervical liquid-based cytology diagnosis≥not clear diagnosis of atypical squamous cells(ASCUS),and the detection of HPV DNA were positive while organized the pathological diagnosis(as the gold standard).48 cases of chronic cervicitis,99 cases of cervical intraepithelial neoplasia(CINⅠ level of 42 cases,27 cases of CINⅡ,CINⅢ in 30 cases) and in 12 cases of invasive squamous cell carcinomas(SCC).Immunohistochemistry was used to detected HPV L1 capsid protein and IL-2 expression in cervical lesions at all level.Results HPV L1 in 159 patients with positive expression rate was 47.80%.In chronic cervicitis,the HPV L1 capsid protein positive rate is 62.50%(30/48),CINⅠ is 83.33%(35/42),CINⅡ 37.03%(10/27),CINⅢ 3.3(1/30),SCC 0(0/12).In addition to CINⅢ and SCC,the difference was not statistically significant(P >0.05),other various cervical lesions compared with the difference statistically significant(P <0.05).Of Il-2 in the organization of a variety of cervical disease,the positive expression rate of 64.78%.In chronic cervicitis,CINⅠ,CINⅡ,CINⅢ and SCC group,positive expression rate are 83.33%(40/48),89.30%(37/42),62.97%(17/27),30%(9/30) and 0(0/12),the difference was statistically significant(P <0.05).Conclusion The positive expression rate of HPVL1 capsid protein and IL-2 is decreasing with the evolution and development of cervical lesions.Uterine intraepithelial neoplasia(CIN),the prediction of the biological behavior of precancerous lesions screening and prevention is of great significance.