银杏叶片对单侧输尿管梗阻大鼠肾脏的保护作用

目的：研究银杏叶片对单侧输尿管梗阻（UUO）大鼠肾脏的保护作用。方法：Wistar大鼠随机分为假手术组、UUO模型组、苯那普利组及银杏叶片组，于给药后2周和4周分别测定各组大鼠肾脏指数、左右肾重比、N-乙酰-β-D-氨基葡萄糖苷酶（NAG）酶活性、尿液尿素氮、尿肌酐、血尿素氮、血肌酐及24 h尿蛋白排泄量等指标。结果：给药后2周，银杏叶组大鼠NAG酶活性 ［(18.0±5.4) U•L^-1］、血尿素氮 ［(7.3±1.3) mmol•L^-1］、血肌酐 ［(35.2±8.4) μmol•L^-1］ 及24 h尿蛋白排泄量 ［(9.82±4.42) mg］ 均明显低于模型组 (P<0.05)；给药后4周，银杏叶片组大鼠NAG酶活性 ［(10.3±4.1) U•L^-1］、血尿素氮 ［(8.7±0.9) mol•L^-1］、血肌酐 ［(44.2±6.7) μmol•L^-1］ 及24 h尿蛋白排泄量 ［(10.17±8.42) mg］均较模型组明显降低 (P<0.05)；给予银杏叶片能减轻UUO大鼠肾间质纤维化程度。结论：口服银杏叶片对UUO大鼠肾功能和结构具有明显保护作用。

Protective effect of Ginkgo biloba tablet on rat kidney with unilateral ureteral obstruction

Objective To investigate the protective effect of Ginkgo biloba(Gb) tablet on rat kidney following unilateral ureteral obstruction(UUO).Methods Wistar rats were divided into four groups: sham group,UUO model group,Gb tablet treatment group and benazapril treatment group(n=12).After Gb tablet was given to UUO rats for 2 and 4 weeks,all rats were sacrificed,the index of kidney,ratio of left and right kidney weight,the activity of urinary N-acetyl-beta-D-glucosaminidase(NAG),urea nitrogen in urine and serum,creatinine in urine and serum and the 24 h urinary protein excretion were detected,respectively.Results The NAG activity [(18.0±5.4) U·L-1],serum urea nitrogen [(7.3±1.3) mmol·L-1],serum creatinine [(35.2±8.4) μmol·L-1] and the 24 h urinary protein excretion [(9.82±4.42) mg] in Gb tablet treatment group were decreased significantly compared with UUO model group(P<0.05) 2 weeks after administration.The NAG activity [(10.3±4.1) U·L-1],serum urea nitrogen [(8.7±0.9) mmol·L-1],serum creatinine [(44.2±6.7) μmol·L-1] and the 24 h urinary protein excretion [(10.17±8.42) mg] in Gb tablet treatment group were obviously lowered than those in UUO model group after treated with Gb for 4 weeks(P<0.05).Gb tablet could lighten the lesion of kidney in UUO rats.Conclusion Gb tablet has obviously protective effect on renal function and structure in UUO rats.