广寄生和瘤果槲寄生及其寄主植物中齐墩果酸、熊果酸含量的相关性研究

目的:考察寄主及其寄生植物齐墩果酸和熊果酸含量的关系.方法:采用HPLC法对寄主及其寄生齐墩果酸、熊果酸含量进行测定,样品采用乙醇超声提取30 min,用Agilent Eclipse XDB-C18柱(250 mm×4.6 mm,5 μm),甲醇-乙腈-0.05%醋酸铵溶液(12:67:21)为流动相,流速为1.0 ml·min-1,检测波长为210 nm.结果:齐墩果酸和熊果酸的线性范围分别为2.61～209.00 mg·L-1(r=0.999 8)和7.85～628.00 mg·L-1(r=0.999 9),浓度与峰面积呈良好线性关系,平均加样回收率分别为99.27%和99.75%.结论:广寄生不含齐墩果酸和熊果酸,瘤果槲寄生中含有齐墩果酸,不含熊果酸.

Correlation Analysis on Contents of Oleanolic Acid and Ursolic Acid among Taxillus chinensis,Viscum ovalifolium DC.and their Host Plants

Objective： To analyze the content correlation of oleanolic acid and ursolic acid among Taxillus chinensis,Viscum ovalifolium DC.and their host plants.Method： Oleanolic acid and ursolic acid were extracted with ethanol solution by ultrasonic for 30 minutes,and the contents were determined by HPLC.An Agilent C18 column（250 mm×4.6 mm,5 μm）was used for the determination with a flow rate of 1.0 ml·min-1 and the column temperature of 25℃.The mobile phase was methanol-acetonitrile-0.05% solution of ammonium acetate（12∶ 67∶ 21） and the detection wavelength was 210 nm.Result： There were good linearity within the range of 2.61-209.00 mg·L-1（r=0.999 8）for oleanolic acid and 7.85-628.00 mg·L-1（r=0.999 9） for ursolic acid with the average recovery of 97.27% and 99.75%,respectively.Conclusion： Both oleanolic acid and ursolic acid can＇t be detected in Taxillus chinensis,while oleanolic acid can be detected in Viscum ovalifolium DC.