HPLC法测定复方莪术油栓中莪术醇的含量

目的:建立高效液相色谱法测定复方莪术油栓中莪术醇含量的方法.方法:采用高效液相色谱法测定,色谱柱为Agilent C18(150 mm×4.6 mm,5 μm),以乙腈-0.2%醋酸溶液(55:45)为流动相,流速为1.0 ml·min-1,柱温为35℃,检测波长为203 nm,进样量为20 μl.结果:莪术醇的浓度在10.36～518.20 μg·ml-1范围内线性关系良好(r=1.000 0),平均回收率为106.92%,RSD=1.86%(n=9).结论:本方法简便、快捷、灵敏度高、专属性强、重复性好,可作为复方莪术油栓中莪术醇含量的测定方法.

Determination of Curcumol in Compound Zedoary Turmeric Oil Suppositories by HPLC

Objective： To establish an HPLC method for the determination of curcumol in compound zedoary turmeric oil suppositories.Method： HPLC was used as the assay method,the column was an Agilent C18（150 mm ×4.6 mm,5 μm） column with the mobile phase of ethanenitrile-0.2% acetic acid（55∶ 45） and the flow rate was 1.0 ml·min-1.The column temperature was 35℃ and the detection wavelength was at 203 nm with injection volume of 20 μl.Result： Curcumol had a good linearity within the range of 10.36~518.20 μg·ml-1（r=1.000 0）.The average recovery was 106.92% with RSD of 1.86%（n=9）.Conclusion： The method is simple,fast,sensitive,reproducible and specific.It can be used in the determination of curcumol in compound zedoary turmeric oil suppositories.